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Series GSE50544 Query DataSets for GSE50544
Status Public on Nov 04, 2013
Title Tudor domain containing 12 (TDRD12) is essential for secondary piRNA biogenesis in mice
Organisms Bombyx mori; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Piwi-interacting RNAs (piRNAs) are gonad-specific small RNAs that provide defence against transposable genetic elements called transposons. Our knowledge of piRNA biogenesis is sketchy, partly due to an incomplete inventory of the factors involved. Here, we identify Tudor domain-containing 12 (TDRD12; also known as ECAT8) as a novel piRNA biogenesis factor in mice. TDRD12 is detected in complexes containing MILI (PIWIL2), its associated primary piRNAs, and TDRD1, all of which are already implicated in secondary piRNA biogenesis. Male mice carrying either a nonsense point mutation (repro23 mice) or a targeted deletion in the Tdrd12 locus are infertile, and de-repress retrotransposons. We find that TDRD12 is dispensable for primary piRNA biogenesis but essential for production of secondary piRNAs that enter MIWI2 (PIWIL4). Cell culture studies with the insect orthologue of TDRD12 suggest a role for the multi-domain protein in mediating complex formation with other participants during secondary piRNA biogenesis.
Overall design Total small RNA and immunoprecipitated small RNA were purified from mouse testis extract and Bmn4 cells for preparation of high-throughput sequencing libraries.
Contributor(s) Pandey RR, Tokuzawa Y, Yang Z, Hayashi E, Ichisaka T, Kajita S, Asano Y, Kunieda T, Sachidanandam R, Chuma S, Yamanaka S, Pillai RS
Citation(s) 24067652
Submission date Sep 03, 2013
Last update date May 15, 2019
Contact name Ramesh Pillai
Organization name University of Geneva
Department Department of Molecular Biology
Street address 30, Quai Ernest-Ansermet
City Gneveva
ZIP/Postal code CH-1211
Country Switzerland
Platforms (2)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL15780 Illumina Genome Analyzer IIx (Bombyx mori)
Samples (12)
GSM1220982 MILI IP #rep1a
GSM1220983 MILI IP #rep1b
GSM1220984 MILI IP #rep2a
BioProject PRJNA217917
SRA SRP029442

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Supplementary file Size Download File type/resource
GSE50544_RAW.tar 1.3 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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