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| Status |
Public on Jul 16, 2003 |
| Title |
Eucalyptus grandis BC progeny |
| Platform organisms |
Eucalyptus grandis; Eucalyptus tereticornis |
| Sample organism |
Eucalyptus grandis |
| Experiment type |
Expression profiling by array
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| Summary |
The gene expression profiles of the differentiating xylem of 91 Eucalyptus grandis backcross individuals were characterized following a loop design (Churchill, G.A. Nat Genet. 2002 Dec;32 Suppl:490-5). In this design, RNA from genotype 1666 (labeled with Cy5) was hybridized with RNA from genotype 1667 (labeled with Cy3) on the first slide(GEO accession number GSM7637); the same genotype 1667 (now labeled with Cy5) was compared with genotype 1669 (Cy3) on the second slide (GSM7638), and so on. The loop was completed when genotype 1666 (Cy3) was contrasted to individual 1796 (Cy3) on slide GSM7727. Therefore, 91 individuals (genotypes) from the E. grandis backcross population were analyzed in two replicates, one with RNA labeled with Cy3 and the other with Cy5.
Keywords = Eucalyptus, xylem, microarray
Keywords: ordered
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| Contributor(s) |
Kirst M, Sederoff R |
| Citation(s) |
15299141, 15687266 |
| Submission date |
Jul 01, 2003 |
| Last update date |
Feb 23, 2012 |
| Contact name |
Matias Kirst |
| E-mail(s) |
mkirst@ufl.edu
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| Phone |
352-8460900
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| Organization name |
University of Florida
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| Department |
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| Street address |
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32611 |
| Country |
USA |
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| Platforms (1) |
| GPL348 |
Eucalyptus xylem microarray (NCSU) |
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| Samples (91)
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| Relations |
| BioProject |
PRJNA85215 |
| Supplementary data files not provided |
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