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Series GSE50140 Query DataSets for GSE50140
Status Public on Mar 08, 2018
Title Renal tubular Sirt1 attenuates diabetic albuminuria by epigenetically suppressing Claudin-1 overexpression in podocytes.
Organism Mus musculus
Experiment type Expression profiling by array
Summary We aim to investigate the role of renal Sirt1 on albuminuria. To further development of our gene expression approach to biodosimetry, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish between wild type(C57BL/6J) mice treated with streptozotocin (WT+STZ) and Sirt1-overexpressing transgenic mice treated with streptozotocin (TG+STZ) mice.
To elucidate the molecular mechanisms underlying the amelioration of albuminuria by our TG (Sirt1-overexpressing transgenic) mice, we performed DNA microarray analysis using micro-dissected glomerular regions to examine the differences in gene expression between WT+STZ and TG+STZ mice.
Overall design Mice in the WT+STZ and TG+STZ groups were killed at 32weeks of age. We dissected the glomerular regions. Micro-dissected glomeruli were immediately used for total RNA isolation and subjected to analysis on microarray chips.
Contributor(s) Hasegawa K
Citation(s) 24141423
Submission date Aug 23, 2013
Last update date Mar 08, 2018
Contact name Kazuhiro Hasegawa
Organization name Tokushima University Graduate School of Biomedical Sciences
Department Department of Nephrology
Street address 3-18-15 Kuramoto-cho
City Tokushima
ZIP/Postal code 770-8503
Country Japan
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (2)
GSM1214656 WT+STZ
GSM1214657 TG+STZ
BioProject PRJNA216144

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50140_RAW.tar 10.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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