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| Status |
Public on Mar 08, 2018 |
| Title |
Renal tubular Sirt1 attenuates diabetic albuminuria by epigenetically suppressing Claudin-1 overexpression in podocytes. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We aim to investigate the role of renal Sirt1 on albuminuria. To further development of our gene expression approach to biodosimetry, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish between wild type(C57BL/6J) mice treated with streptozotocin (WT+STZ) and Sirt1-overexpressing transgenic mice treated with streptozotocin (TG+STZ) mice.
To elucidate the molecular mechanisms underlying the amelioration of albuminuria by our TG (Sirt1-overexpressing transgenic) mice, we performed DNA microarray analysis using micro-dissected glomerular regions to examine the differences in gene expression between WT+STZ and TG+STZ mice.
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| Overall design |
Mice in the WT+STZ and TG+STZ groups were killed at 32weeks of age. We dissected the glomerular regions. Micro-dissected glomeruli were immediately used for total RNA isolation and subjected to analysis on microarray chips.
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| Contributor(s) |
Hasegawa K |
| Citation(s) |
24141423 |
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| Submission date |
Aug 23, 2013 |
| Last update date |
Mar 08, 2018 |
| Contact name |
Kazuhiro Hasegawa |
| E-mail(s) |
kazuhiro@tokushima-u.ac.jp
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| Organization name |
Tokushima University Graduate School of Biomedical Sciences
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| Department |
Department of Nephrology
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| Street address |
3-18-15 Kuramoto-cho
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| City |
Tokushima |
| ZIP/Postal code |
770-8503 |
| Country |
Japan |
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| Platforms (1) |
| GPL10333 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA216144 |
