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Series GSE49689 Query DataSets for GSE49689
Status Public on Dec 11, 2013
Title A Mesodermal Factor, T (BRACHYURY), specifies mouse germ cell fate by directly activating germline determinants.
Organism Mus musculus
Experiment type Expression profiling by array
Summary The germ cell lineage ensures reproduction and heredity in metazoans. Primordial germ cells (PGCs) in mice are induced in pluripotent epiblast cells by BMP4 and WNT3, yet their mechanism of action remains elusive. Here, using in vitro PGC specification system, we show that WNT3, but not BMP4, induces many transcription factors associated with mesoderm in epiblast-like cells (EpiLCs) through beta-CATENIN. Among these, T (BRACHYURY), a classical and conserved mesodermal factor, was essential for robust activation of Blimp1 and Prdm14, two of the germline determinants. T, but not SMAD1 or beta-CATENIN/TCF1, binds distinct regulatory elements of both Blimp1 and Prdm14, and directly up-regulates these genes without BMP4 and WNT3. Without BMP4, a program induced by WNT3 prevents T from activating Blimp1 and Prdm14, demonstrating that BMP4 is permissive for PGC specification. These findings establish a fundamental role of a mesodermal gene in PGC specification, a potentially evolutionarily conserved mechanism across metazoans.
 
Overall design Wnt3 (+/+) and Wnt3 (-/-) mouse embryonic stem cells (mESCs) bearing the BV transgenes expressing membrane-targeted Venus under the control of Blimp1 regulatory elements (Blimp1-mVenus: BV; PMID 18583473) were established and maintained. Epiblast-like cells (EpiLCs) were then induced from the established ES lines, followed by the cytokine stimulation (No cytokines [negative control], BMP4, Wnt3a, BMP4+Wnt3a) for different periods (0h, 12h, 24h, 36h).
 
Contributor(s) Aramaki S, Hayashi K, Kurimoto K, Ohta H, Yabuta Y, Iwanari H, Mochizuki Y, Hamakubo T, Shirahige K, Saitou M
Citation(s) 24331926
Submission date Aug 08, 2013
Last update date Feb 11, 2019
Contact name Kazuki Kurimoto
E-mail(s) kurimoto@naramed-u.ac.jp
Organization name Nara Medical University
Department School of medicine
Lab Department of Embryology
Street address 840 Shijo-Cho, Kashihara
City Nara
ZIP/Postal code 634-8521
Country Japan
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (23)
GSM1204920 Wnt3+/+_0h
GSM1204921 Wnt3-/-_0h
GSM1204922 Wnt3+/+_non_12h
Relations
BioProject PRJNA214738

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49689_RAW.tar 140.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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