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Series GSE49668 Query DataSets for GSE49668
Status Public on Mar 08, 2014
Title Small RNA content in mouse serum upon S.mansoni infection
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary MicroRNAs (miRNAs) are a class of short non-coding RNA that play important roles in disease processes in animals and are present in a highly stable cell-free form in body fluids. Here we examine the capacity of host and parasite miRNAs to serve as tissue or serum biomarkers of Schistosoma mansoni infection.
Sequencing of small RNAs from serum confirmed the presence of miRNAs and revealed 11 parasite-derived miRNAs that were detectable by 8 weeks post S.mansoni infection.
Overall design Small RNA content in serum of naïve and Schistosoma mansoni infected mice were examined in two different librarys. 1- prepared according to the 290 Illumina small RNA Sample Preparation Kit version 1.5 and sequenced on the GAIIX and 2- prepared according to the TruSeq Small RNA protocol (without size-selecting small 295 RNA) and sequenced on the HiSeq2
Contributor(s) Buck AH, Hoy AM, Quintana JF, Ivens A
Citation(s) 24587461
Submission date Aug 08, 2013
Last update date May 15, 2019
Contact name Amy Buck
Organization name The University of Edinburgh
Street address West Mains Road
City Edinburgh
ZIP/Postal code EH9 3JT
Country United Kingdom
Platforms (2)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (4)
GSM1204715 7weeks_naive
GSM1204716 7weeks_infected
GSM1204717 8weeks_naive
BioProject PRJNA214686
SRA SRP028609

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49668_RAW.tar 1.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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