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Series GSE49507 Query DataSets for GSE49507
Status Public on Mar 02, 2014
Title Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub
Organism Mus musculus
Experiment type Expression profiling by array
Summary The aim of the dataset was to study on a genome-wide level the impact of Lat deficiency on gene expression in resting and activated CD4+ T cells
Overall design Lat+ and Lat− CD4+ T cells were isolated from lymph nodes and spleen of Latfl-dtr Tmat-Cre mice using a Dynabeads untouched mouse CD4 cells kit (Life Technology) and further purified by cell sorting. Lat+ CD4+ T cells were defined as: CD5+, hDTR+, CD8−, TCRγδ−, CD25−, CD69−, CD62L+, lineage (CD11c, CD11b, CD19, CD45R, CD161)− and Lat− CD4+ Tcells were defined as: CD5+, hDTR−, CD8−, TCRγδ−, CD25−, CD69−, CD62L+, lineage (CD11c, CD11b, CD19, CD45R, CD161)−. Sorted Lat+ or Lat− CD4+ T cells were then kept in vitro for 4 hours without stimulation or activated for 4 hours using anti-CD3 antibody and anti-CD28 antibody. Cell samples corresponding to three biological replicates were analyzed and gene expression profiles were obtained from total RNA.
Contributor(s) Roncagalli R, Hauri S, Fiore F, Liang Y, Chen Z, Sansoni A, Kanduri K, Joly R, Malzac A, Lähdesmäki H, Lahesmaa R, Yamasaki S, Saito T, Malissen M, Aebersold R, Gstaiger M, Malissen B
Citation(s) 24584089
Submission date Aug 02, 2013
Last update date Apr 18, 2017
Contact name Kartiek Kanduri
Phone +358-2-333-8001
Organization name Turku Centre for Biotechnology
Lab Molecular Systems Immunology / Lahesmaa
Street address Tykistokatu 6
City Turku
ZIP/Postal code 20520
Country Finland
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (12)
GSM1200175 LymphNode_Spleen_CD4+ T cells_LAT+_rep1
GSM1200176 LymphNode_Spleen_CD4+ T cells_LAT+_rep2
GSM1200177 LymphNode_Spleen_CD4+ T cells_LAT+_rep3
BioProject PRJNA214354

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Supplementary file Size Download File type/resource
GSE49507_RAW.tar 49.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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