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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 02, 2014 |
| Title |
Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of the dataset was to study on a genome-wide level the impact of Lat deficiency on gene expression in resting and activated CD4+ T cells
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| Overall design |
Lat+ and Lat− CD4+ T cells were isolated from lymph nodes and spleen of Latfl-dtr Tmat-Cre mice using a Dynabeads untouched mouse CD4 cells kit (Life Technology) and further purified by cell sorting. Lat+ CD4+ T cells were defined as: CD5+, hDTR+, CD8−, TCRγδ−, CD25−, CD69−, CD62L+, lineage (CD11c, CD11b, CD19, CD45R, CD161)− and Lat− CD4+ Tcells were defined as: CD5+, hDTR−, CD8−, TCRγδ−, CD25−, CD69−, CD62L+, lineage (CD11c, CD11b, CD19, CD45R, CD161)−. Sorted Lat+ or Lat− CD4+ T cells were then kept in vitro for 4 hours without stimulation or activated for 4 hours using anti-CD3 antibody and anti-CD28 antibody. Cell samples corresponding to three biological replicates were analyzed and gene expression profiles were obtained from total RNA.
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| Contributor(s) |
Roncagalli R, Hauri S, Fiore F, Liang Y, Chen Z, Sansoni A, Kanduri K, Joly R, Malzac A, Lähdesmäki H, Lahesmaa R, Yamasaki S, Saito T, Malissen M, Aebersold R, Gstaiger M, Malissen B |
| Citation(s) |
24584089 |
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| Submission date |
Aug 02, 2013 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Kartiek Kanduri |
| Phone |
+358-2-333-8001
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| Organization name |
Turku Centre for Biotechnology
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| Lab |
Molecular Systems Immunology / Lahesmaa
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| Street address |
Tykistokatu 6
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| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM1200175 |
LymphNode_Spleen_CD4+ T cells_LAT+_rep1 |
| GSM1200176 |
LymphNode_Spleen_CD4+ T cells_LAT+_rep2 |
| GSM1200177 |
LymphNode_Spleen_CD4+ T cells_LAT+_rep3 |
| GSM1200178 |
LymphNode_Spleen_CD4+ T cells_LAT+_Act_4h_rep1 |
| GSM1200179 |
LymphNode_Spleen_CD4+ T cells_LAT+_Act_4h_rep2 |
| GSM1200180 |
LymphNode_Spleen_CD4+ T cells_LAT+_Act_4h_rep3 |
| GSM1200181 |
LymphNode_Spleen_CD4+ T cells_LAT__rep1 |
| GSM1200182 |
LymphNode_Spleen_CD4+ T cells_LAT__rep2 |
| GSM1200183 |
LymphNode_Spleen_CD4+ T cells_LAT__rep3 |
| GSM1200184 |
LymphNode_Spleen_CD4+ T cells_LAT__Act_4h_rep1 |
| GSM1200185 |
LymphNode_Spleen_CD4+ T cells_LAT__Act_4h_rep2 |
| GSM1200186 |
LymphNode_Spleen_CD4+ T cells_LAT__Act_4h_rep3 |
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| Relations |
| BioProject |
PRJNA214354 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49507_RAW.tar |
49.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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