|
| Status |
Public on Nov 25, 2013 |
| Title |
Genome-wide acetylated histone 3 lysine 9 (H3K9ac) in HDAC3-depleted liver rescued with deacectylase-dead HDAC3 mutants |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We found that several deacetylase-dead HDAC3 mutants were able to rescue the metabolic phenotype of HDAC3-depleted livers. Here we profile the histone acetylation in the presence of different HDAC3 mutants in mouse liver.
|
| |
|
| Overall design |
Deacetylase-dead HDAC3 mutants, including HAHA, KA, YF and HEBI, were introduced into HDAC3-depleted (Cre) mouse livers by virus along with wild-type (WT) HDAC3 as a control. Livers were harvested at 5 pm (ZT 10) and subjected to ChIP with anti-H3K9ac antibodies followed by deep sequencing.
|
| |
|
| Contributor(s) |
Fang B, Feng D, Sun Z, Lazar MA |
| Citation(s) |
24268577 |
| Submission date |
Jul 30, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Bin Fang |
| E-mail(s) |
binfang@mail.med.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Lab |
Mitch Lazar
|
| Street address |
3400 Civic Center Blvd, Bldg 42
|
| City |
Philadelphia |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA213727 |
| SRA |
SRP028329 |
Less...
More...