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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 16, 2014 |
| Title |
INO80 complex in the core regulatory network governing ESC self-renewal [771_timecourse] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Embryonic stem cell (ESC) self-renewal and pluripotency is controlled by the coordinated action of transcription factors and chromatin regulators. Compared to the pluripotency transcription factors, the function of the chromatin regulators, especially the ATP-dependent chromatin remodelers, remains poorly understood in ESCs. Here, we show that INO80, a SWI/SNF family chromatin remodeling complex, is essential for ESC self-renewal, pluripotency, somatic cell reprogramming, and embryonic development. Ino80, the ATPase of the complex, forms an auto-regulatory loop with the ESC master transcription factors Oct4, Nanog, and Sox2. More importantly, it co-occupies the enhancer regions of most key pluripotency genes with the master transcription factors, and positively regulates their expression by maintaining an open chromatin structure. Our data suggests that INO80 is an integral component of the pluripotency transcription network, and plays a critical role in both the maintenance and establishment of pluripotency
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| Overall design |
Mouse J1 cells were infected with lentiviral-shNT or lentiviral-Ino80, RNA was extracted at 2, 4 or 6 days after infection.
agent: non-targeting shRNA: NS_Day_2_1, NS_Day_2_2, NS_Day_4_1, NS_Day_4_2, NS_Day_6_1, NS_Day_6_2
agent: Ino80 shRNA: shIno80_Day_2_1, shIno80_Day_2_2, shIno80_Day_4_1, shIno80_Day_4_2, shIno80_Day_6_1, shIno80_Day_6_2
time: 48 hours: NS_Day_2_1, NS_Day_2_2, shIno80_Day_2_1, shIno80_Day_2_2
time: 96 hours: NS_Day_4_1, NS_Day_4_2, shIno80_Day_4_1, shIno80_Day_4_2
time: 144 hours: NS_Day_6_1, NS_Day_6_2, shIno80_Day_6_1, shIno80_Day_6_2
cell line: mouse J1 ESC: NS_Day_2_1, NS_Day_2_2, shIno80_Day_2_1, shIno80_Day_2_2, NS_Day_4_1, NS_Day_4_2, shIno80_Day_4_1, shIno80_Day_4_2, NS_Day_6_1, NS_Day_6_2, shIno80_Day_6_1, shIno80_Day_6_2
biological replicate: shIno80_Day_2_1, shIno80_Day_2_2
biological replicate: shIno80_Day_4_1, shIno80_Day_4_2
biological replicate: shIno80_Day_6_1, shIno80_Day_6_2
biological replicate: NS_Day_2_1, NS_Day_2_2
biological replicate: NS_Day_4_1, NS_Day_4_2
biological replicate: NS_Day_6_1, NS_Day_6_2
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| Contributor(s) |
Wang L, Hu G, Ward JM |
| Citation(s) |
24792115 |
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| Submission date |
Jul 26, 2013 |
| Last update date |
May 10, 2018 |
| Contact name |
Li Wang |
| Organization name |
NIH
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| Street address |
111 T.W. Alexander Dr
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| City |
Durham |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE49252 |
INO80 complex in the core regulatory network governing ESC self-renewal |
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| Relations |
| BioProject |
PRJNA213417 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49250_RAW.tar |
116.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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