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Series GSE49194 Query DataSets for GSE49194
Status Public on Oct 01, 2013
Title Expression data from neurospheres derived from the neocortex, striatum and subventricular zones of the adult mouse brain
Organism Mus musculus
Experiment type Expression profiling by array
Summary Differential gene expression profiles of neurospheres derived from different regions of the adult brain.
In this dataset, we include data on triplicated biological samples from each of the following brain regions: neocortex, striatum, and subventricular zone. We also include duplicated data on neurospheres derived from the dorsal and ventralforebrain neural tubes and one spinal cord of E14.5 mouse embryos for comparison.
 
Overall design Fourteen samples were analyzed in total. To compare the expression profiles across the samples, Affymetrix GeneChip Mouse Genome 430 2.0 Array Platform (total 41,170 probes) was used, and the data were analyzed using Silicon Genetics GeneSpring Software GX7.3.1. The data was normalized to median settings across the entire probe set and samples, and transcripts that are expressed across triplicated samples at levels two-fold or higher than the rest of the samples were considered as significantly enriched in particular biological samples. Comparisons between adult and embryonic samples also identified genes enriched in adult brain-derived samples.
 
Contributor(s) Grande A, Nakafuku M
Citation(s) 23974433
Submission date Jul 25, 2013
Last update date Feb 11, 2019
Contact name Masato Nakafuku
Organization name Cincinnati Children's Hospital Medical Center
Department Developmental Biology
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (14)
GSM1195268 Adult neocortex sample 1
GSM1195269 Adult neocortex sample 2
GSM1195270 Adult neocortex sample 3
Relations
BioProject PRJNA213267

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49194_RAW.tar 54.6 Mb (http)(custom) TAR (of CEL)
GSE49194_matrix_ExpressionAnalysis_MetricsTab.txt.gz 2.7 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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