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| Status |
Public on Jun 02, 2006 |
| Title |
Time course microarray data following GR activation in MCF10A-Myc breast cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This series contain time course microarray data from MCF10A-Myc cells treated with either ethanol or Dexamethasone for 30 min, 2 hr, 4 hr, and 24 hr. This series contains three biological replicates that were analyzed as independent replicate experiments (data were normalized within each replicate experiment, not across all samples).
Keywords: time course
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| Overall design |
MCF10A-Myc cells were subjected to 72 hrs of growth factor withdrawal followed by treatment with either vehicle (ethanol) or Dex (10-6 M) for 0.5, 2, 4 or 24 hrs. Total RNA from each sample was extracted using Qiagen’s RNeasy Kit. Preparation of biotinylated cRNA and hybridization to oligonucleotide arrays (Affymetrix human genome genechip HG-U133A) were performed at the University of Chicago Microarray Core Facility. HG-U133A genechip contains 22,215 probe sets that represent approximately 16,000 human genes. The complete procedure was repeated independently three times. Gene chips were scanned and analyzed using Robust Multi-array Average (RMA) algorithm.
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| Contributor(s) |
Min Z, Wu W, Brickley D, Pew T, Conzen S |
| Citation(s) |
16690749 |
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| Submission date |
May 24, 2006 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Suzanne Daniela Conzen |
| E-mail(s) |
sconzen@medicine.bsd.uchicago.edu
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| Phone |
(773)834-2604
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| Organization name |
The University of Chicago
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| Department |
Medicine
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| Lab |
Conzen Lab
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| Street address |
5841 S. Maryland Ave, SBRI J301, MC 2115
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (24)
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| GSM109204 |
Control(ethanol)-treated sample at 30min, biological rep1 |
| GSM109205 |
Dex-treated sample at 30min, biological rep1 |
| GSM109206 |
Control(ethanol)-treated sample at 2hr, biological rep1 |
| GSM109207 |
Dex-treated sample at 2hr, biological rep1 |
| GSM109208 |
Control(ethanol)-treated sample at 4hr, biological rep1 |
| GSM109209 |
Dex-treated sample at 4hr, biological rep1 |
| GSM109210 |
Control(ethanol)-treated sample at 24hr, biological rep1 |
| GSM109211 |
Dex-treated sample at 24hr, biological rep1 |
| GSM109212 |
Control(ethanol)-treated sample at 30min, biological rep2 |
| GSM109213 |
Dex-treated sample at 30min, biological rep2 |
| GSM109214 |
Control(ethanol)-treated sample at 2hr, biological rep2 |
| GSM109215 |
Dex-treated sample at 2hr, biological rep2 |
| GSM109216 |
Control(ethanol)-treated sample at 4hr, biological rep2 |
| GSM109217 |
Dex-treated sample at 4hr, biological rep2 |
| GSM109218 |
Control(ethanol)-treated sample at 24hr, biological rep2 |
| GSM109219 |
Dex-treated sample at 24hr, biological rep2 |
| GSM109220 |
Control(ethanol)-treated sample at 30min, biological rep3 |
| GSM109221 |
Dex-treated sample at 30min, biological rep3 |
| GSM109222 |
Control(ethanol)-treated sample at 2hr, biological rep3 |
| GSM109223 |
Dex-treated sample at 2hr, biological rep3 |
| GSM109224 |
Control(ethanol)-treated sample at 4hr, biological rep3 |
| GSM109225 |
Dex-treated sample at 4hr, biological rep3 |
| GSM109226 |
Control(ethanol)-treated sample at 24hr, biological rep3 |
| GSM109227 |
Dex-treated sample at 24hr, biological rep3 |
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| Relations |
| BioProject |
PRJNA95859 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE4917_RAW.tar |
85.0 Mb |
(http)(custom) |
TAR (of CEL) |
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