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| Status |
Public on Jul 18, 2013 |
| Title |
Expression data of TAGLN-overexpressing RKO human colon cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Transgelin was the top-ranked marker of metastatic potential identified in the comparison of node-positive colorectal cancer (CRC) versus node-negative CRC in our previous study. Transgelin is localized in the nucleus of cultured CRC cells and microRNA-mediated knockdown of TAGLN (the gene encoding transgelin) expression modulates the expression of genes involved in the epithelial-to-mesenchymal transition.
We performed gene expression profiling on control and transgelin-overexpressing RKO cells using Affymetrix microarray technology.
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| Overall design |
The plasmid carrying TAGLN was generated using the pcDNA6.2/EmGFP-Bsd/V5-DEST vector (Invitrogen, Carlsbad, CA) and the pDONR221-TAGLN-mut plasmid from a previous study by an LR recombination reaction (http://www.invitrogen.com). After lipofectamine-mediated transfection of the TAGLN-carrying plasmid or the vector-only control plasmid, stable transfectants were selected and cultured in medium containing 25 μg/ml blasticidin followed by RNA extraction and hybridization on Affymetrix microarrays. Six samples were obtained from three independent experiments (technical replicates).
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| Contributor(s) |
Lin Y, Fang Y, Zhou H |
| Citation(s) |
26847345, 32774160 |
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| Submission date |
Jul 17, 2013 |
| Last update date |
Sep 08, 2020 |
| Contact name |
Ying Lin |
| E-mail(s) |
linwy@mail.sysu.edu.cn
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| Organization name |
Sun Yat-sen Memorial Hospital, Sun Yat-sen Univeristy
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| Department |
Gastroenterology and Hepatology
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| Street address |
107 West Yanjiang Road
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510120 |
| Country |
China |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| GSM1191939 |
TAGLN-overexpressing RKO cells, technical rep1 |
| GSM1191940 |
TAGLN-overexpressing RKO cells, technical rep2 |
| GSM1191941 |
TAGLN-overexpressing RKO cells, technical rep3 |
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| Relations |
| BioProject |
PRJNA212543 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48998_RAW.tar |
25.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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