|
| Status |
Public on Jul 10, 2014 |
| Title |
C1q elicited macrophage gene expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We have data from a previous microarray experiment which shows that C1q elicits macrophages to express genes important for clearance of dead (apoptotic) cells. In this experiment we want to determine the signaling mechanism by which C1q is turning on macrophage gene expression. For our control (sample 1-3) we heated C1q to denature it so it is inactive. We also want to know which portion of C1q is responsible for activation the macrophage so we generated C1qtails (samples 4-5). We also have macrophages treated with the whole C1q molecule (7-9). And finally we have a cell line (J774) treated with C1q but does not activate the cells (samples 10-12).
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| Overall design |
Bone marrow derived macrophages or J774 cells were adhered to heat inactivated C1q, C1q tails or C1q for 18 hours. Cells were harvested and RNA was isolated and converted to double stranded cDNA and subsequently labeled and hybridized onto NimbleGen mouse gene expression array [100718_MM9_EXP] using Nimblegen Hybridization system 4 according to manufacturer’s instructions (Roche)
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| Contributor(s) |
Galvan M, Foreman D, Zeng E, Tan J, Bohlson S |
| Citation(s) |
24942043 |
| |
| Submission date |
Jul 09, 2013 |
| Last update date |
Oct 22, 2019 |
| Contact name |
Erliang Zeng |
| E-mail(s) |
ezeng@nd.edu
|
| Organization name |
University of Notre Dame
|
| Street address |
384 Fitzpatrick Hall
|
| City |
Notre Dame |
| State/province |
IN |
| ZIP/Postal code |
46556 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL15887 |
NimbleGen Mouse gene expression array [100718_MM9_EXP] (gene-level version) |
|
| Samples (12)
|
| GSM1182447 |
Mouse_macrophage_stimulated_with_heat_inactivated_C1q_control_rep1 |
| GSM1182448 |
Mouse_macrophage_stimulated_with_heat_inactivated_C1q_control_rep2 |
| GSM1182449 |
Mouse_macrophage_stimulated_with_heat_inactivated_C1q_control_rep3 |
| GSM1182450 |
Mouse_macrophage_stimulated_with_C1qtail_tail_portion_of_C1q_protein_rep1 |
| GSM1182451 |
Mouse_macrophage_stimulated_with_C1qtail_tail_portion_of_C1q_protein_rep2 |
| GSM1182452 |
Mouse_macrophage_stimulated_with_C1qtail_tail_portion_of_C1q_protein_rep3 |
| GSM1182453 |
Mouse_macrophage_stimulated_with_C1q_whole_molecule_rep1 |
| GSM1182454 |
Mouse_macrophage_stimulated_with_C1q_whole_molecule_rep2 |
| GSM1182455 |
Mouse_macrophage_stimulated_with_C1q_whole_molecule_rep3 |
| GSM1182456 |
Mouse_macrophage_cell_line_J774_stimulated_with_C1q_whole_molecule_rep1 |
| GSM1182457 |
Mouse_macrophage_cell_line_J774_stimulated_with_C1q_whole_molecule_rep2 |
| GSM1182458 |
Mouse_macrophage_cell_line_J774_stimulated_with_C1q_whole_molecule_rep3 |
|
| Relations |
| BioProject |
PRJNA210847 |
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