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| Status |
Public on Mar 20, 2014 |
| Title |
Fate changes leading to multipotency of isolated mesenchymal cells [Expression: Population_vs_Clone] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mesenchymal populations include a fraction of cells exhibiting multipotency as well as others with limited differentiation range. It has been assumed that the mesenchymal cellular cascade is topped by a multipotent cell, which gives rise to progeny with diminishing differentiation potentials. Here we show that cultured mesenchymal cells, a priori exhibiting a limited differentiation potential, may gain new capacities and become multipotent following single cell isolation. These fate changes were accompanied by up-regulation of differentiation promoting genes, many of which also became H4K20me1 methylated. Early events in the process included TGFβ and Wnt modulation, and down-regulation of hypoxia signaling. Indeed, hypoxic conditions inhibited the observed cell changes. Overall, cell isolation from neighboring partners caused major molecular changes and particularly, a newly established epigenetic state. It is suggested that MSCs behave non-deterministically and non-hierarchically and should therefore be defined primarily by their capacity to undergo fate changes triggered by environmental cues.
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| Overall design |
The gene expression profile of MSC population was compared with one of its derived single cell clones
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| Contributor(s) |
Shoshani O, Leshkowitz D, Massalha H, Ravid O, Zipori D |
| Citation(s) |
24715711 |
| |
| Submission date |
Jul 04, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Ofer Shoshani |
| E-mail(s) |
ofsher@hotmail.com
|
| Organization name |
Ludwig Institute for Cancer Research
|
| Lab |
Don Cleveland
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE48547 |
Fate changes leading to multipotency of isolated mesenchymal cells |
|
| Relations |
| BioProject |
PRJNA210557 |
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