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| Status |
Public on Jul 04, 2013 |
| Title |
Restoration of anterior regeneration in a planarian with limited regenerative ability |
| Organism |
Procotyla fluviatilis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Variability of regenerative potential among animals has long perplexed biologists. Based on their amazing regenerative abilities, planarians have become important models for understanding the molecular basis of regeneration; however, planarian species with limited regenerative abilities are also found. Despite the importance of understanding the differences between closely related, regenerating and non-regenerating organisms, few studies have focused on the evolutionary loss of regeneration, and the molecular mechanisms leading to such regenerative loss remain obscure. Here we examine Procotyla fluviatilis, a planarian with restricted ability to replace missing tissues, utilizing next-generation sequencing to define the gene expression programs active in regeneration-permissive and regeneration-deficient tissues. We found that Wnt signaling is aberrantly activated in regeneration-deficient tissues. Remarkably, down-regulation of canonical Wnt signaling in regeneration-deficient regions restores regenerative abilities: blastemas form and new heads regenerate in tissues that normally never regenerate. This work reveals that manipulating a single signaling pathway can reverse the evolutionary loss of regenerative potential.
RNA-seq experiments to identify gene expression changes following amputation in body regions with variable regenerative potential.
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| Overall design |
Adult Procotyla fluviatilis were amputated at sites either anterior or posterior to the pharynx. After 24 hours post-amputation, tissues near the amputation site were excised and RNA was extracted. Similar tissues were excised from uncut control animals. Samples were processed for RNA-seq using Illumina procedures.
We generated a de novo P. fluviatilis transcriptome and used RNA sequencing (RNA-seq) to characterize transcripts from excised tissue fragments in Reg+ and Reg- body regions 24 hours post-amputation. We performed parallel analyses on tissues excised from intact animals at identical body regions to account for regional differences in transcripts, thereby identifying changes resulting from amputation.
Samples A1-A3 = Regeneration-proficient (Reg+) tissue excision 24 hours after amputation.
Samples B1-B3 = Tissue excision from regeneration-proficient (Reg+) region but not amputated.
Samples C1-C3 = Tissue excision from regeneration-deficient (Reg-) tissues 24 hours after amputation.
Samples D1, D3-D4 = Tissue excision from regeneration-deficient (Reg-) region that was not amputated.
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| Contributor(s) |
Sikes JM, Newmark PA |
| Citation(s) |
23883929 |
| BioProject |
PRJNA205293 |
| |
| Submission date |
Jul 02, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
James Sikes |
| E-mail(s) |
jsikes@usfca.edu
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| Phone |
4154226367
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| Organization name |
University of San Francisco
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| Department |
Biology
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| Street address |
2130 Fulton Street
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94117 |
| Country |
USA |
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| Platforms (1) |
| GPL17405 |
Illumina HiSeq 2000 (Procotyla fluviatilis) |
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| Samples (12)
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| Relations |
| SRA |
SRP023959 |
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