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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 22, 2013 |
| Title |
Inducing pluripotency from mouse somatic cells by small-molecule compounds [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource for regenerative medicine. However, genetic manipulation and difficult-to-manufacture strategies used in reprogramming limit their clinical applications. Here, we show pluripotency can be induced from mouse somatic cells by specific small-molecule compounds. The completely chemically-induced pluripotent stem cells (CiPSCs) can be stably maintained in embryonic stem cell (ESC) culture medium and resemble ESCs in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. These findings suggest that exogenous master genes are dispensable for cell fate reprogramming and pave the way for the clinical application of somatic reprogramming techniques. Chemicals' acronym: V, VPA; C, CHIR; 6, 616452; T, tranylcypromine; F, FSK; Z, DZNep; P, PGE2; R, RG108; S, SRT1720; M, 2-Me-5HT; D, D4476; B, Sodium butyrate.
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| Overall design |
mRNA expression analysis of mouse embryonic fibroblasts (MEFs), GFP- cells, GFP+ clusters,GFP+ colonies, embryonic stem cells (ESCs) and CiPSCs by RNA sequencing.
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| Contributor(s) |
Hou P, Li Y, Zhang X, Liu C, Guan J, Li H, Zhao Y, Deng H |
| Citation(s) |
23868920 |
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| Submission date |
Jun 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
chun liu |
| E-mail(s) |
dabai22222@163.com
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| Phone |
13621043040
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| Organization name |
Peking University
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| Street address |
beijing
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| City |
beijing |
| State/province |
beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE48243 |
Inducing pluripotency from mouse somatic cells by small-molecule compounds |
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| Relations |
| SRA |
SRP026281 |
| BioProject |
PRJNA209700 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48252_rnaseq_processed.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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