 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 01, 2013 |
| Title |
Microarray analysis of elp2 and wild type (Col-0) infected with the necrotrophic fungal pathogen Botrytis cinerea |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of the microarray experiment was to identify defense genes that were differentially expressed in the Arabidopsis mutant elp2 and wild type in response to infection of the necrotrophic fungal pathogen Botrytis cinerea. Results indicated that, compared with the wild type, the WRKY33/OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2/ERF59 (ORA59)/ETHYLENE RESPONSE FACTOR1 transcriptional cascades are down-regulated, whereas the MYC2 transcriptional cascade is up-regulated in the elp2 mutant.
|
| |
|
| Overall design |
Three biological replicates with leaves from 8-12 plants per sample were collected at 0, 3, 6, 12, 24, and 48 hours after inoculation with the necrotrophic fungal pathogen Botrytis cinerea. After extraction, RNA concentration was determined on a NanoDrop Spectrophotometer (Thermofisher Scientific, Waltham, MA) and sample quality was assessed using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Equal amount of RNA from the 3 biological replicates were used for microarray analysis. The channels of the dual-channel arrays were analyzed independently.
|
| |
|
| Contributor(s) |
Wang C, Ding Y, Yao J, Zhang Y, Sun Y, Mou Z |
| Citation(s) |
23607369 |
| |
| Submission date |
Jun 21, 2013 |
| Last update date |
Apr 22, 2015 |
| Contact name |
Zhonglin Mou |
| Organization name |
University of Florida
|
| Department |
Microbiology and Cell Science & Plant Molecular and Cellular Biology
|
| Street address |
981 Museum Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL12621 |
Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Probe Name version) |
|
| Samples (40)
|
|
| Relations |
| BioProject |
PRJNA209201 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48207_RAW.tar |
625.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...