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| Status |
Public on Oct 01, 2013 |
| Title |
Different DNA methylation between hypothalamus paraventricular (PVH) and ventromedial (VMH) nuclei |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal is to compare the DNA methylation patterns between ARH and PVH to examine to what extent DNA methylation is region specific in genome scale.
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| Overall design |
ARH and PVH were micro-dissected from mice brains. Each 7 pieces of ARH or PVH were pooled and used for DNA methylation comparison by methylation specific amplification microarray (MSAM). As a brief description of the MSAM: 500ng of genomic DNA was serially digested with SmaI and XmaI followed by an adaptor ligation and adaptor mediated PCR amplification and then cohybridization. Two-color cohybridizations were performed as paired comparison of PVH vs. ARH with two biological replicates including dye swap.
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| Contributor(s) |
Zhang W, Matalan-Hung N, Simerly RB, Waterland RA |
| Citation(s) |
24186871 |
| |
| Submission date |
Jun 21, 2013 |
| Last update date |
Dec 04, 2013 |
| Contact name |
Ge Li |
| Organization name |
Baylor College of Medicine
|
| Department |
Pediatrics
|
| Lab |
Robert A. Waterland's lab
|
| Street address |
1100 Bates St.
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL17331 |
Agilent-017202 whole mouse genome (mm8) custom 2x105k array |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE48323 |
DNA methylation in hypothalamus |
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| Relations |
| BioProject |
PRJNA209715 |
