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Series GSE47978 Query DataSets for GSE47978
Status Public on Jun 15, 2013
Title EWI-2 regulates melanoma growth and lung metastasis in a TGF-β1-dependent manner
Organism Homo sapiens
Experiment type Expression profiling by array
Summary EWI-2 (IgSF8) plays a novel, bifunctional role in melanoma cells. EWI-2 inhibits migration, metastasis, EMT-like changes, and CD271-dependent invasion in multiple melanoma cell lines. On the other hand, EWI-2 supports melanoma cell proliferation, survival, and xenograft growth. Consistent with these results, EWI-2 levels were elevated in human malignant melanoma, but not in metastatic melanoma samples. Altered melanoma cell functions, caused by EWI-2 ablation, are almost entirely dependent on enhanced TRF-β1 signaling, and also require contributions from tetraspanin proteins CD9 and CD81. In melanoma cells lacking EWI-2, tetraspanins CD9 and CD81 enhance TRF-β1 signaling by facilitating TβR-2−TβR-1 receptor complex formation. When EWI-2 is present, CD9 and CD81 are diverted into EWI-2 complexes, and thus TRF-β1 signaling is inhibited.
Overall design 4 samples = 2 Control + 2 EWI-2 KD.
Contributor(s) Wang H, Sharma C, Knoblich K, Granter SR, Hemler ME
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Submission date Jun 14, 2013
Last update date Dec 06, 2018
Contact name Konstantin Knoblich
Organization name Monash University
Department Biochemistry and Molecular Biology
Street address 15 Innovation Walk
City Clayton
State/province VIC
ZIP/Postal code 3800
Country Australia
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (4)
GSM1163978 Control cells 1
GSM1163979 Control cells 2
GSM1163980 EWI-2 KD cells 1
BioProject PRJNA208517

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47978_EWI2KD.gct.txt.gz 1.2 Mb (ftp)(http) TXT
GSE47978_RAW.tar 8.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Processed data are available on Series record

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