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| Status |
Public on Jun 15, 2013 |
| Title |
EWI-2 regulates melanoma growth and lung metastasis in a TGF-β1-dependent manner |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
EWI-2 (IgSF8) plays a novel, bifunctional role in melanoma cells. EWI-2 inhibits migration, metastasis, EMT-like changes, and CD271-dependent invasion in multiple melanoma cell lines. On the other hand, EWI-2 supports melanoma cell proliferation, survival, and xenograft growth. Consistent with these results, EWI-2 levels were elevated in human malignant melanoma, but not in metastatic melanoma samples. Altered melanoma cell functions, caused by EWI-2 ablation, are almost entirely dependent on enhanced TRF-β1 signaling, and also require contributions from tetraspanin proteins CD9 and CD81. In melanoma cells lacking EWI-2, tetraspanins CD9 and CD81 enhance TRF-β1 signaling by facilitating TβR-2−TβR-1 receptor complex formation. When EWI-2 is present, CD9 and CD81 are diverted into EWI-2 complexes, and thus TRF-β1 signaling is inhibited.
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| Overall design |
4 samples = 2 Control + 2 EWI-2 KD.
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| Contributor(s) |
Wang H, Sharma C, Knoblich K, Granter SR, Hemler ME |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 14, 2013 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Konstantin Knoblich |
| Organization name |
Monash University
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| Department |
Biochemistry and Molecular Biology
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| Lab |
STIMMLAB
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| Street address |
15 Innovation Walk
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| City |
Clayton |
| State/province |
VIC |
| ZIP/Postal code |
3800 |
| Country |
Australia |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA208517 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47978_EWI2KD.gct.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE47978_RAW.tar |
8.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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