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| Status |
Public on Jun 07, 2013 |
| Title |
Osteotropism in breast cancer metastasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Breast cancer metastases develop in the bone more frequently than any other site, and are a common cause of morbidity in the form of bone pain, pathological fractures, nerve compression, and life-threatening hypercalcemia. Despite ongoing research efforts, the molecular and cellular mechanisms that regulate breast cancer cell homing to and colonization of the bone as well as resultant pathological bone alteration remain poorly understood. To identify key mediators promoting breast cancer metastasis to bone, we utilized an immunocompetent, syngeneic murine model of breast cancer metastasis employing the mammary tumor cell line NT2.5. Following intracardiac injection of NT2.5 cells in neu-N mice, metastases developed in the bone, liver, and lung, closely mimicking the anatomical distribution of metastases in breast cancer patients. Using an in vivo selection process, we established NT2.5 sub-lines demonstrating an enhanced ability to colonize the bone and liver. Genome-wide cDNA microarray analysis comparing gene expression between parental NT2.5 cells and established sub-lines was performed.
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| Overall design |
Individual samples of RNA from parental NT2.5 cells, early passage (BO3 and LI1) sub-lines, and final passage (BO6 and LI3) sub-lines were compared using GeneChip® Mouse Genome 430 2.0 Arrays
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| Contributor(s) |
Kominsky SL |
| Citation(s) |
19642106, 23861746 |
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| Submission date |
Jun 06, 2013 |
| Last update date |
Feb 07, 2020 |
| Contact name |
Scott Kominsky |
| E-mail(s) |
kominsc@jhmi.edu
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| Organization name |
Johns Hopkins University School of Medicine
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| Street address |
720 Rutland Ave, Ross 209
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA207351 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47714_RAW.tar |
21.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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