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| Status |
Public on Sep 16, 2015 |
| Title |
small RNAnext Generation Sequencing of bone marrow-derived macrophages treated in M-CSF withdrawal medium |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The goal of this study is to investigate the presence of novel small RNAs in apoptotic macrophage
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| Overall design |
Method: cultured mouse (bl6 line) bone marrow were differentiated in for 7 days in conditioned medium, in preence of Macrophage-Colony Stimulating Factor (M-CSF). M-CSF also stimulates the survive of macrophages by activating PI3K/AKT pathway. In accordance to previous publication (Lombardo, E., Alvarez-Barrientos, A., Maroto, B., Bosca, L., and Knaus, U.G, 2007, TLR4-mediated survival of macrophages is MyD88 dependent and requires TNF-alpha autocrine signalling, J Immunol 178, 3731-3739) apoptosis was induced in culturaed bone marrow-derived macrophages upon 36 hr of M-CSF withdrawal. Total RNA was isolated and a small RNA library was made using "Small RNA sequencing kit" (Illumina), according to the manufacturer’s instructions.
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| Contributor(s) |
Trabucchi M, Tanasa B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 28, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE47609 |
Atherogenic Stimuli Trigger Ago2-hnRNP A1-Dependent Processing of RNYs Regulating Apoptosis and Inflammatory Response in Macrophages |
|
| Relations |
| BioProject |
PRJNA206057 |
| SRA |
SRP023489 |
