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| Status |
Public on Jan 09, 2014 |
| Title |
Inducible expression of MyoD directly mediates myogenic conversion of human induced pluripotent stem cells (iPSCs) derived from Duchenne muscular dystrophy (DMD). |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Genome-wide gene expression analysis of MyoD-infected DMD-specific iPSCs (GM05112-M5.1) on days 0 (untreated), day 3 and day 8 post Dox treatment, human primary myoblasts (undifferentiated and as differentiated myotubes), and undifferentiated iPSCs from healthy donors (iPSCs-1 and iPSCs-2).
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| Overall design |
DMD-specific iPSCs were infected with lentivirus expressing MyoD under the control of Tet-inducible promoter and another lentivirus expressing the transactivator. To initiate myogenic differentiation, iPSCs were treated with 1µg/ml Dox. RNA was isolated 0, 3 and 8 days later and gene expression analysis was performed.
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| |
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| Contributor(s) |
Abujarour R, Robbins DL, Flynn P |
| Citation(s) |
24396035 |
| |
| Submission date |
May 03, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Dave Robbins |
| E-mail(s) |
dave.robbins@fatetherapeutics.com
|
| Organization name |
Fate Therapeutics
|
| Street address |
3535 General Atomics Court Ste. 200
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (7)
|
| GSM1133454 |
iPSCs from healthy donor (on feeders) |
| GSM1133455 |
iPSCs from healthy donor (feeder-free) |
| GSM1133456 |
MyoD-infected DMD-specific iPSCs (GM05112-M5.1), untreated (feeder-free) |
| GSM1133457 |
MyoD-infected DMD-specific iPSCs (GM05112-M5.1), day 3 post-dox addition |
| GSM1133458 |
MyoD-infected DMD-specific iPSCs (GM05112-M5.1), day 8 post-dox addition |
| GSM1133459 |
Human skeletal muscle myoblasts, in expansion media |
| GSM1133460 |
Human skeletal muscle myoblasts, in differentiation media (day 3) |
|
| Relations |
| BioProject |
PRJNA201337 |
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