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Status |
Public on May 30, 2013 |
Title |
Bivalent Chromatin Marks Developmental Regulatory Genes in the Mouse Embryonic Germline in Vivo |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We developed a ChIP protocol for the analysis of histone marks using less than 10,000 cells per IP, and used it to investigate the chromatin state of E11.5 mouse primordial germ cells (PGCs). A genome-wide ChIP-Seq analysis of E11.5 PGCs revealed a distribution of H3K4me3/H3K27me3 bivalent domains highly enriched for developmental regulatory genes.
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Overall design |
H3K4me3 and H3K27me3 ChIP-Seq from mouse E11.5 primordial germ cells.
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Contributor(s) |
Sachs M, Onodera CS, Blaschke K, Ebata KT, Song JS, Ramalho-Santos M |
Citation(s) |
23727241 |
Submission date |
Apr 25, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Courtney Onodera |
E-mail(s) |
courtney.onodera@ucsf.edu
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Organization name |
University of California, San Francisco
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Street address |
1450 Third St, HD374
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City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94158 |
Country |
USA |
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Platforms (1) |
GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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Samples (3) |
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Relations |
BioProject |
PRJNA199926 |
SRA |
SRP021522 |