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Series GSE46396 Query DataSets for GSE46396
Status Public on May 30, 2013
Title Bivalent Chromatin Marks Developmental Regulatory Genes in the Mouse Embryonic Germline in Vivo
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We developed a ChIP protocol for the analysis of histone marks using less than 10,000 cells per IP, and used it to investigate the chromatin state of E11.5 mouse primordial germ cells (PGCs). A genome-wide ChIP-Seq analysis of E11.5 PGCs revealed a distribution of H3K4me3/H3K27me3 bivalent domains highly enriched for developmental regulatory genes.
Overall design H3K4me3 and H3K27me3 ChIP-Seq from mouse E11.5 primordial germ cells.
Contributor(s) Sachs M, Onodera CS, Blaschke K, Ebata KT, Song JS, Ramalho-Santos M
Citation(s) 23727241
Submission date Apr 25, 2013
Last update date May 15, 2019
Contact name Courtney Onodera
Organization name University of California, San Francisco
Street address 1450 Third St, HD374
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (3)
GSM1129743 PGC_Input_ChIPSeq
GSM1129744 PGC_H3K4me3_ChIPSeq
GSM1129745 PGC_H3K27me3_ChIPSeq
BioProject PRJNA199926
SRA SRP021522

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Supplementary file Size Download File type/resource
GSE46396_RAW.tar 600.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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