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Series GSE46202 Query DataSets for GSE46202
Status Public on Aug 08, 2013
Title Coordination of engineered factors with TET1/2 promotes early stage epigenetic modification during somatic cell reprogramming
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Somatic cell reprogramming towards induced pluripotent stem cells (iPSCs) holds great promise in future regenerative medicine, however, the reprogramming process mediated by the traditional defined factors (OSNK) is slow and extremely inefficient. Here we show that a combination of modified reprogramming factors (OySyNyK), in which the transactivation domain of the Yes-associated protein is fused to OCT4, SOX2 and NANOG respectively, could be used to establish a highly efficient and rapid reprogramming system for iPSC generation. We show that the efficiency of OySyNyK-induced iPSCs was up to 100-fold higher than that of induction by traditional OSNK. Moreover we show that the reprogramming by OySyNyK is very rapid (initiated at 24 h versus 5 d by OSNK). Compared with OSNK, we found that OySyNyK factors significantly increased the expression of Tet1/2 at the early stage, which will interact with OSNK factors, and co-occupy pluripotency loci in the genome for somatic cell reprogramming. Our studies not only establish a rapid and highly efficient iPSC reprogramming system, but also uncover a novel mechanism by which OSNK factors coordinate with TET proteins to regulate 5hmC-mediated epigenetic control, thereby promoting somatic cell reprogramming.
Overall design We examined DNA hydroxymethylation progression of reprogramming intermediates. To this end, we profiled the genome-wide 5hmC distribution in MEFs, the reprogramming intermediates at different stages induced by either the OSNK or OySyNyK methods, and iPSCs using the chemical capture approach
Contributor(s) Li Y, Wang T
Citation(s) 24672749
Submission date Apr 18, 2013
Last update date Feb 03, 2020
Contact name Tao Wang
Phone 4047270405
Organization name Emory University
Department Human Genetics
Lab Warren Lab
Street address 615 Michael Street
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (8)
GSM1126190 MEFP3
GSM1126191 SKOND1
GSM1126192 SKOND3
BioProject PRJNA198029
SRA SRP021183

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Supplementary file Size Download File type/resource
GSE46202_RAW.tar 9.3 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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