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Series GSE45897 Query DataSets for GSE45897
Status Public on Apr 10, 2013
Title Gene expression in the islets of 12 wk old Non-Obese Diabetic (NOD) mice and healthy control NOD.B10 mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Gene expression in the islets of NOD and NOD.B10 mice at 12 weeks of age, prior to the onset of destructive insulitis.
Overall design The pancreata of NOD and NOD.B10 mice (n=6) were frozen and islets were isolated by laser capture microdissection. Cryosections (8 μm) were cut and stained using the Arcturus HistoGene Frozen Section Staining kit (Applied Biosystems) and laser dissection was performed using the Leica AS LMD and the Leica IM 1000 Image Manager Basic software. Sections from at least 40 individual islets were collected and RNA was extracted using the RNeasy micro kit (Qiagen). RNA quality was assessed using the Agilent 2100 Bioanalyzer and the RNA 6000 Pico Reagent Kit (Agilent). Samples were preamplified using the TrueLabeling-PicoAMP kit (SA Biosciences), post-labeled with Cy5 and run against a Cy3-labeled mouse Universal RNA control (SA Biosciences). Microarrays were performed using the Whole Mouse Genome Microarray Kit, 4×44K 2-color arrays (Agilent Technologies).
Contributor(s) Yip L
Citation(s) 24264405
Submission date Apr 09, 2013
Last update date Jan 12, 2017
Contact name Linda Yip
Organization name Stanford University
Department Medicine
Lab C.G. Fathman
Street address 269 Campus Drive West, CCSR Building Rm. 2240
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (12)
GSM1119300 NOD Islet - sample 1
GSM1119301 NOD Islet - sample 2
GSM1119302 NOD Islet - sample 3
BioProject PRJNA196617

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45897_RAW.tar 48.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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