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Series GSE45407 Query DataSets for GSE45407
Status Public on Jul 15, 2014
Title Gene expression of hormone-treated U2OS cells expressing GR alleles
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Glucocorticoid receptor (GR) is a hormone-activated, DNA-binding transcriptional regulatory factor, which regulates diverse aspects of physiology. GR recognizes specifically imperfect palindromic six base pair “half sites
separated by thoursee base pair “spacers”, binding as an inverted dimer. In vivo, different GR target genes depend on different functional surfaces of GR for proper regulation, but rules dictating the relationship between
sequence and utilization of distinct GR functional surfaces remain unknown.In this study, we measured changes in gene expression and genomic occupancy upon glucocorticoid treatment of isogenic cultured human
cell lines expressing alleles of GR bearing differences in particular functional surfaces.
Overall design Total RNA was isoalted from U2OS cells expressing GRalpha, GRgamma, A477T, 30iiB, or E773R after treatment with 100 nM dexamethasone for 2, 4, or 24 hours, or ethanol for 4 hours (0 hours).
There are four biological replicates of each condition.
Contributor(s) Schiller BJ, Chondankar R, Watson LC, Stallcup MR, Yamamoto KR
Citation(s) 25085117
Submission date Mar 21, 2013
Last update date Mar 20, 2017
Contact name Benjamin J Schiller
Organization name University of California, San Francisco
Lab Keith Yamamoto
Street address 600 16th St
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (80)
GSM1103820 U2OS_30iiB-0hr-replicate2
GSM1103821 U2OS_GRalpha-4hr-replicate2
GSM1103822 U2OS_GRalpha-2hr-replicate1
BioProject PRJNA193692

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45407_RAW.tar 1.5 Gb (http)(custom) TAR (of TAR)
GSE45407_biological_replicates.txt.gz 346 b (ftp)(http) TXT
GSE45407_non-normalized.txt.gz 7.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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