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Series GSE45371 Query DataSets for GSE45371
Status Public on Feb 11, 2014
Title Absolute and induction TNF tolerance in primary human monocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The molecular basis of TNF tolerance is poorly understood. In this experiment, primary human monocytes were used to examine TNF refractoriness. We detect absolute tolerance as selective, dose-dependently affecting a small group of powerful effector molecules; induction tolerance represented a more general phenomenon.
Overall design Primary human monocytes were incubated for 48 h with Medium or different TNF-doses and subsequently short-term stimulated for two hours with TNF. Total RNA was prepared and analysed by use of Agilent microarrays. 6 different conditions were analyzed in total, corresponding to cells that were: 1) medium incubated (M+M), 2) medium incubated and short time TNF (400 U/ml) incubated (M+T), 3) low dose TNF (40 U/ml) preincubated (40+M), 4) low dose TNF preincubated and short time TNF incubated (40+T), 5) high dose TNF (400 U/ml) preincubated (400+M), 6) high dose TNF preincubated and short time TNF incubated (400+T). 3 biological replicates (corresponding to three different healthy donors) were analyzed regarding high-dose tolerance conditions (preincubation with 400 U TNF/ml), one of which was analyzed in duplicates (including cell culture, RNA preparation and a dye-swap microarray approach). 2 biological replicates (corresponding to two different healthy donors) were analyzed under low-dose tolerance conditions (preincubation with 40 U/ml). The different human donors were numbered and indicated in brackets (d1-d5).
Contributor(s) Günter J, Vogt N, Hampel K, Dittrich-Breiholz O, Bikker R, Page S, Müller B, Kandemir J, Kracht M, Huber R, Brand K
Citation(s) 24574500, 30871024
Submission date Mar 20, 2013
Last update date Apr 17, 2019
Contact name Oliver Dittrich-Breiholz
Organization name Medical School Hannover
Department Research Core Unit Genomics
Street address Carl-Neuberg-Str. 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (12)
GSM1103342 M+T/M+M(d1)
GSM1103343 M+T/M+M(d1replicate)
GSM1103344 400+T/400+M(d1)
BioProject PRJNA193569

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45371_RAW.tar 192.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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