 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 14, 2013 |
| Title |
Loss-of-function of MYO5B induces epithelial cell scattering in enterocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
A non-functional myosin Vb motor in duodenal enterocytes results in disruption of epithelial cell polarity characterized by complete loss of microvilli and mislocalization of apical brush border proteins in the cytoplasm which finally cause a devastating disease in neonates with severe malabsorption defects accompanied by protracted diarrhea during infancy, classified as microvillus inclusion disease (MVID). The exact mechanisms how loss-of-function of MYO5B induces polarity loss are not completely understood in MVID pathogenesis. Obtaining better insights in cell polarity defects caused by loss of MYO5B, we performed microarray- in combination with protein expression-analysis in an inducible CaCo2 MYO5B RNAi cell system. Surprisingly, in MYO5B-depleted CaCo2 cells, CDH1 coding for the cell adhesion protein E-Cadherin and important for cell adhesion and therefore maintenance of cell polarity, was significantly downregulated. Interestingly, mesenchymal cell markers, specifically Vimentin and N-Cadherin, physiologically not expressed in differentiated epithelium, were upregulated and accompanied by increased phospho-c-jun levels in the nucleus. Importantly phospho-c-jun was also found in nuclei of duodenal enterocytes in MVID patients, indicating loss of MYO5B induces epithelial cell scattering in enterocytes.
|
| |
|
| Overall design |
3 Myosin 5B KD versus 3 control samples
|
| |
|
| Contributor(s) |
Thoeni CE, Vogel GF, Bindreither D, Rainer J, Charoentong P, Trajanoski Z, Geley S, Lechner S, Pfaller K, Hess MW, Müller T, Janecke AR, Cutz E, Huber LA |
| Citation(s) |
24138727 |
| |
| Submission date |
Mar 13, 2013 |
| Last update date |
Mar 13, 2014 |
| Contact name |
Daniel Bindreither |
| E-mail(s) |
daniel.bindreither@gmail.com
|
| Organization name |
Medical University Innsbruck
|
| Department |
Division of Molecular Pathophysiology
|
| Lab |
Applied Bioinformatics Group
|
| Street address |
Innrain 82
|
| City |
Innsbruck |
| State/province |
Tirol |
| ZIP/Postal code |
6020 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL16786 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [HuGene10stv1_Hs_ENSG version 13.0.0] |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA192945 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45134_RAW.tar |
30.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...