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Series GSE45127 Query DataSets for GSE45127
Status Public on Mar 14, 2013
Title Aneuploidy, oncogene amplification, and epithelial to mesenchymal transition define spontaneous transformation of murine epithelial cells [transcriptome]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Human epithelial cancers are defined by a recurrent distribution of specific chromosomal aneuploidies. In our model system, mouse bladder and kidney epithelial cells spontaneously immortalize, transform and become tumorigenic after prolonged culture. We assessed genome and transcriptome alterations and found wide-spread aneuploidy, early transcriptional deregulation, and massive genomic dereguation of the cellular transcriptome.
The results reveal a remarkable similarity with genome and transcriptome aberrations detected in human tumorigenesis, hence validating our newly derived cancer models.
 
Overall design Epithelial cells were isolated from the C57BL/6 mouse bladder and kidney. These cells underwent spontaneous transformation in culture. We sought to identify the molecuar genomic alterations that occur during the transformation process and to compare these with the changes observed in human bladder and kidney cancers.
 
Contributor(s) Padilla-Nash HM, McNeil NE, Yi M, Nguyen Q, Hu Y, Wangsa D, Mack D, Hummon AB, Case C, Cardin E, Stevens R, Difilippantonio MJ, Ried T
Citation(s) 23619298
Submission date Mar 13, 2013
Last update date Jun 03, 2013
Contact name Hesed Maria Padilla-Nash
E-mail(s) nashh@mail.nih.gov
Phone 301-435-4019
Organization name NCI
Department Genetics Branch
Lab Section of Cancer Genomics
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892-8010
Country USA
 
Platforms (1)
GPL16763 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array [CDF: Brainarray V.10, Mm430A_Mm_ENTREZG_v10]
Samples (82)
GSM1097662 Female bladder cells pooled, 1 technical replicate 1
GSM1097663 Female bladder cells pooled, 1 technical replicate 2
GSM1097664 Female bladder cells pooled, 2 technical replicate 1
This SubSeries is part of SuperSeries:
GSE45129 Aneuploidy, oncogene amplification, and epithelial to mesenchymal transition define spontaneous transformation of murine epithelial cells
Relations
BioProject PRJNA192939

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Supplementary file Size Download File type/resource
GSE45127_RAW.tar 180.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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