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Series GSE44764 Query DataSets for GSE44764
Status Public on Apr 18, 2013
Title The histone demethylase Jmjd3 sequentially associates with the transcription factors Tbx3 and Eomes to drive endoderm differentiation
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Here we show that T-box proteins team up with chromatin modifying enzymes to drive the expression of the key lineage regulator, Eomes during endodermal differentiation of embryonic stem (ES) cells. The Eomes locus is maintained in a transcriptionally poised configuration in ES cells. During early differentiation steps, the ES cell factor Tbx3 associates with the histone demethylase Jmjd3 at the enhancer element of the Eomes locus to allow enhancer-promoter interactions. This spatial reorganization of the chromatin primes the cells to respond to Activin signaling, which promotes the binding of Jmjd3 and Eomes to its own bivalent promoter region to further stimulate Eomes expression in a positive feedback loop.
Overall design Examination of the binding of pluripotency factors to mouse embryonic stem cells and embryoid bodies
Contributor(s) Kartikasari AE, Zhou JX, Kanji MS, Chan DN, Sinha A, Grapin-Botton A, Magnuson M, Lowry WE, Bhushan A
Citation(s) 23584530
Submission date Mar 01, 2013
Last update date May 15, 2019
Contact name April Kartikasari
Organization name UCLA
Department Medical-Endocrinology
Street address 900 Veteran Avenue
City Los Angeles
ZIP/Postal code 90025
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (4)
GSM1090228 Tbx3 ChIP-Seq 1
GSM1090229 Tbx3 ChIP-Seq 2
GSM1090230 Nanog ChIP-Seq 1
BioProject PRJNA191606
SRA SRP019221

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44764_RAW.tar 469.2 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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