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| Status |
Public on Apr 18, 2013 |
| Title |
The histone demethylase Jmjd3 sequentially associates with the transcription factors Tbx3 and Eomes to drive endoderm differentiation |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Here we show that T-box proteins team up with chromatin modifying enzymes to drive the expression of the key lineage regulator, Eomes during endodermal differentiation of embryonic stem (ES) cells. The Eomes locus is maintained in a transcriptionally poised configuration in ES cells. During early differentiation steps, the ES cell factor Tbx3 associates with the histone demethylase Jmjd3 at the enhancer element of the Eomes locus to allow enhancer-promoter interactions. This spatial reorganization of the chromatin primes the cells to respond to Activin signaling, which promotes the binding of Jmjd3 and Eomes to its own bivalent promoter region to further stimulate Eomes expression in a positive feedback loop.
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| |
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| Overall design |
Examination of the binding of pluripotency factors to mouse embryonic stem cells and embryoid bodies
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| |
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| Contributor(s) |
Kartikasari AE, Zhou JX, Kanji MS, Chan DN, Sinha A, Grapin-Botton A, Magnuson M, Lowry WE, Bhushan A |
| Citation(s) |
23584530 |
| Submission date |
Mar 01, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
April Kartikasari |
| E-mail(s) |
akartikasari@mednet.ucla.edu
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| Organization name |
UCLA
|
| Department |
Medical-Endocrinology
|
| Street address |
900 Veteran Avenue
|
| City |
Los Angeles |
| ZIP/Postal code |
90025 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA191606 |
| SRA |
SRP019221 |
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