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| Status |
Public on Feb 22, 2013 |
| Title |
Identification of genes involved in cell differentiation of rat oral epithelial cell line ROE2 |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
The use of in vitro cell culture systems has been of central importance for research of physiology, pharmacology, and toxicology, and functions at the cellular and molecular levels. We have developed an immortalized oral epithelial cell line ROE2 from fetal transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen. Here, to identify genes involved in ROE2 cell differentiation, global-scale gene expression analysis was carried out using a GeneChip® system.
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| Overall design |
ROE2 cells, an immortalized oral epithelial cell line from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen, were cultured for 0, 3, 6 and 9 days at 37˚C. Total RNA samples were prepared from the cells, and quality of the RNA was analyzed using a Bioanalyzer 2100. Gene expression was monitored by an Affymetrix GeneChip® system with a Rat Genome 230 2.0 array. Sample preparation for array hybridization was carried out as described in the manufacturer's instructions.
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| Contributor(s) |
Tabuchi Y, Kariya A, Wada S |
| Citation(s) |
24521861 |
| |
| Submission date |
Feb 21, 2013 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Yoshiaki Tabuchi |
| E-mail(s) |
ytabu@cts.u-toyama.ac.jp
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| Organization name |
University of Toyama
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| Department |
Life Science Research Center
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| Lab |
Division of Molecular Genetics Research
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| Street address |
2630 Sugitani
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| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA190056 |
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