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Series GSE44426 Query DataSets for GSE44426
Status Public on Jan 10, 2015
Title Transcriptome analysis of bone marrow mesenchymal stromal cells from patients with Primary Myelofibrosis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Primary myelofibrosis (PMF) is a clonal myeloproliferative neoplasm whose severity and treatment complexity is attributed to the presence of bone marrow (BM) fibrosis and alterations of stroma impairing the production of normal blood cells. Despite the recently discovered mutations including the JAK2V617F mutation in about half of patients, the primitive event responsible for the clonal proliferation is still unknown. In the highly inflammatory context of PMF, the presence of fibrosis associated with a neoangiogenesis and an osteosclerosis concomitant to the myeloproliferation and to the increase number of circulating hematopoietic progenitors suggests that the crosstalk between hematopoietic and stromal cells is deregulated in the PMF BM microenvironment. Within these niches, Mesenchymal Stromal Cells (BM-MSC) play a supportive role in the production of growth factors and extracellular matrix which regulate the proliferation, differentiation, adhesion and migration of hematopoietic progenitors. A transcriptome analysis of BM-MSC in PMF patients will help to characterize their molecular alterations and to understand their involvement in the hematopoietic progenitor deregulation that features PMF.
Primary Myelofibrosis, mesenchymal stroma cells, bone marrow, myeloproliferative disorders
 
Overall design Transcriptome analysis was performed on BM-MSC amplified in vitro after 3 to 5 passages. Agilent Whole Human Genome Oligo Microarrays were used to compare expression profiling of BM-MSC from PMF patients and healthy donors.
 
Contributor(s) Martinaud C, Desterke C, Konopacki J, Vannucchi AM, Guglielmelli P, Boutin L, Lataillade J, Le Bousse-Kerdil├Ęs M
Citation(s) 25840601
Submission date Feb 20, 2013
Last update date Feb 22, 2018
Contact name christophe desterke
E-mail(s) christophe.desterke@inserm.fr
Phone +33 (0)145595316
Organization name university Paris Sud11
Department UFR medecine institute Andre Lwoff
Lab hopital Paul Brousse Batiment Lavoisier
Street address 14-16 avenue Paul Vaillant Couturier
City VILLEJUIF
ZIP/Postal code 94807
Country France
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (12)
GSM1084994 control 1
GSM1084995 control 2
GSM1084996 control 3
Relations
BioProject PRJNA189948

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44426_RAW.tar 115.4 Mb (http)(custom) TAR (of TXT)
GSE44426_median_normalized.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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