NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE44382 Query DataSets for GSE44382
Status Public on Feb 20, 2013
Title Expression data from Bifidobacterium animalis ssp. lactis strains exposed to hydrogen peroxide stress
Platform organism Bifidobacterium animalis subsp. lactis
Sample organisms Bifidobacterium animalis subsp. lactis DSM 10140; Bifidobacterium animalis subsp. lactis Bl-04
Experiment type Expression profiling by array
Summary Stress survival tactics in bacteria utilize the up- and down-regulation of stress response genes. In bacteria that lack classical stress response genes for oxidative stress, other cellular systems can be used for cell survival.
We used custom microarrays to study the regulation of genes in Bifidobacterium animalis ssp. lactis strains to oxidative stress to elucidate novel stress response mechanisms.
 
Overall design Bifidobacterium cells were grown to late log phase then harvested and exposed to a sub-lethal level of hydrogen peroxide. Samples were taken at 5 and 20 mins for RNA extraction and hybridization on Affymetrix microarrays. Controls were prepared for each time point which recieved no hydrogen peroxide treatment.
 
Contributor(s) Oberg TS, Broadbent JR
Citation(s) 23772066
Submission date Feb 19, 2013
Last update date Sep 06, 2013
Contact name Taylor S Oberg
E-mail(s) taylor.oberg@aggiemail.usu.edu
Organization name Utah State University
Department NDFS
Lab Broadbent
Street address 8700 Old Main
City Logan
State/province UT
ZIP/Postal code 84322
Country USA
 
Platforms (1)
GPL16692 [Blactisa520677F] Custom Affymetrix Bifidobacteria Genome Array [gene-level]
Samples (24)
GSM1084111 BL-04 unexposed control cells at 0 min, biological rep1
GSM1084112 BL-04 unexposed control cells at 0 min, biological rep2
GSM1084113 BL-04 unexposed control cells at 0 min, biological rep3
Relations
BioProject PRJNA189708

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44382_RAW.tar 12.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap