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Series GSE4392 Query DataSets for GSE4392
Status Public on Sep 22, 2006
Title Expression data from native ZAP-70+CD38+ vs. ZAP-70-CD38- CLL cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary B-cell chronic lymphocytic leukemia (B-CLL) is a heterogenous disease with a highly variable clinical course and analysis of ZAP-70 and CD38 expression on B-CLL cells allowed for identification of patients with good (ZAP-70-CD38-), intermediate (discordant expression of ZAP-70 and CD38) and poor (ZAP-70+CD38+) prognosis. In an attempt to identify a molecular basis that may underly this diverse clinical behaviour DNA microarray technology was employed to compare eight ZAP-70+CD38+ with eight ZAP-70-CD38- B-CLL cases.
We used microarrays to detail the global programme of gene expression distinguising B-CLL from patient with good (samples 1 to 8) and poor prognosis (sample 9 to 16) and identified distinct classes of up- and down-regulated genes.
Keywords: Disease progression
Overall design To compare the transcriptosomes of good prognosis CLL cases (ZAP-70-CD38-) to poor prognosis cases (ZAP-70+CD38+), we purified CD19+ cells from peripheral blood samples by immunomagnetic isolation using MidiMacs, resulting in >95% purity of leukemic cells as detected by FACS analysis of CD19+CD5+ cells. The leukemic cells were freshly purified from untreated patients and RNA was directly isolated from fresh cells without further ex vivo treatment of the cells. Eight immunomagnetically purified peripheral blood derived ZAP-70+CD38+ CLL cases were compared with eight ZAP-70-CD38- B-CLL cases.
Contributor(s) Huettmann A, Klein-Hitpass L, Thomale J, Deenen R, Carpinteiro A, Nueckel H, Edelmann J, Sellmann L, Duehrsen U, Duerig J
Citation(s) 16932341
Submission date Mar 07, 2006
Last update date Aug 10, 2018
Contact name Ludger Klein-Hitpass
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (16)
GSM99339 B-CLL sample 653, good prognosis
GSM99340 B-CLL sample 623, good prognosis
GSM99341 B-CLL sample 813, good prognosis
BioProject PRJNA94581

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