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| Status |
Public on Dec 13, 2013 |
| Title |
ChIP-Seq of transcriptional regulators in MM1S cells upon small molecule treatment. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Discovery of the genome-wide location of proteins using ChIP-Seq has allowed global mapping of the key transcription factors and chromatin regulators that control gene expression programs in various cells. Many DNA-associated processes are targeted for disease therapy. This study investigates the functions of small molecule therapeutics that target DNA-associated processes involved of CDK9 and BRD4.
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| Overall design |
Genomic DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. ChIP was performed using an antibody against RNAP2, BRD2, BRD3, BRD4, CDK8, CDK9, H3K27me3, H3K20me3, and CTCF using whole cell extract (WCE) as a background control.
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| |
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| Contributor(s) |
Lars A, Hoke H, Peter R, Young RA |
| Citation(s) |
24336317 |
| |
| Submission date |
Jan 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard A Young |
| E-mail(s) |
young_computation@wi.mit.edu
|
| Phone |
617-258-5219
|
| Organization name |
Whitehead Institute for Biomedical Research
|
| Lab |
Young Lab
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA187203 |
| SRA |
SRP018149 |
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