|
Status |
Public on Jun 30, 2016 |
Title |
RNA-sequencing (RNA-seq) in breast cancer cell lines after ectopic manipulation of miR-26a expression |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
RNA sequencing technology has been carried out in order to evaluate mRNA expression changes after manipulation of miR-26a in both MCF-7 and MDA-MB-231 breast cancer cell lines.
|
|
|
Overall design |
To evaluate the entire set of genes modulated by miR-26a in breast cancer, we performed RNA-seq after ectopic manipulation of this miRNA. We over-expressed miR-26a in MCF-7 epithelial cancer cell lines and also reduced its activity by stably transfecting MDA-MB-231 mesenchymal-like cancer cell lines with a specific sponge vector. GO terms and pathway enriched analysis of the transcripts that significantly change upon miR-26 ectopic manipulation implicates miR-26ab in cell cycle, apoptosis, cell spreading and cell adhesion in breast cancer
|
|
|
Citation(s) |
28126920 |
|
Submission date |
Jan 24, 2013 |
Last update date |
Feb 21, 2023 |
Contact name |
Leandro Castellano |
E-mail(s) |
l.castellano@sussex.ac.uk
|
Organization name |
University of Sussex
|
Street address |
Falmer, JMS Building
|
City |
Brighton |
ZIP/Postal code |
BN1 9QG |
Country |
United Kingdom |
|
|
Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
Samples (4)
|
|
Relations |
BioProject |
PRJNA187476 |
SRA |
SRP018221 |