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Series GSE43539 Query DataSets for GSE43539
Status Public on Mar 02, 2013
Title H3K4me3 Interactions with TAF3 Regulate Preinitiation Complex Assembly and Selective Gene Activation [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Histone modifications regulate chromatin-dependent processes, yet the mechanisms by which they contribute to specific outcomes remain unclear. H3K4me3 is a prominent histone mark that is associated with active genes and promotes transcription through interactions with effector proteins that include initiation factor TFIID. We demonstrate that H3K4me3-TAF3 interactions direct global TFIID recruitment to active genes, some of which are p53 targets. Further analyses show that (i) H3K4me3 enhances p53-dependent transcription by stimulating preinitiation complex (PIC) formation; (ii) H3K4me3, through TAF3 interactions, can act either independently or cooperatively with the TATA box to direct PIC formation and transcription; and (iii) H3K4me3-TAF3/TFIID interactions regulate gene-selective functions of p53 in response to genotoxic stress. Our findings indicate a mechanism by which H3K4me3 directs PIC assembly for the rapid induction of specific p53 target genes
Overall design Examination of TAF3, RNAPII, and H3K4me3 distribution in HCT116 cells.
Contributor(s) Lauberth SM, Nakayama T, Wu X, Ferris A, Hughes SH, Roeder RG
Citation(s) 23452851
Submission date Jan 16, 2013
Last update date May 15, 2019
Contact name Xiaolin Wu
Phone 301-846-7677
Organization name Frederick National Laboratory for Cancer Research/SAIC-Frederick Inc
Department Cancer Research Technology Program
Lab Genomics Laboratory
Street address 8560 Progress Drive, C3001
City Frederick
State/province MD
ZIP/Postal code 21701
Country USA
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (4)
GSM1065007 Input DNA
GSM1065008 TAF3_ChIPSeq
GSM1065009 H3K4me3_ChIPSeq
This SubSeries is part of SuperSeries:
GSE43542 H3K4me3 Interactions with TAF3 Regulate Preinitiation Complex Assembly and Selective Gene Activation
BioProject PRJNA186696
SRA SRP017967

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43539_RAW.tar 370.8 Mb (http)(custom) TAR (of BED, BW, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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