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| Status |
Public on Nov 01, 2013 |
| Title |
Polysome-associated mRNA profiling of cancer cells in response to CXCL12 and IGF1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CXCL12 and IGF1 are key secreting molecules produced by cancer-associated fibroblasts in breast cancer. These factors promote the survival of disseminated cancer cells in the bone marrow. To assess the combined responses elicited by CXCL12 and IGF1, we examined the translating transcriptome of cancer cells in response to these two factors by Translating Ribosome Affinity Purification (TRAP)-RNAseq.
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| Overall design |
MDA-MB-231 cells were engineered to express an EGFP-tagged version of ribosomal protein L10a. This allows the retrieval of polysome-associated mRNA by anti-GFP pull down (TRAP) and profiling the translating transcriptome by RNAseq. EGFP-L10a+ cancer cells were serum starved (0.2% serum) for 24 hours, and then treated with CXCL12 (30ng/mL) + IGF1 (10ng/mL) or CXCL12 (300ng/mL) + IGF1 (100ng/mL) for 6hrs. Two biological replicates were profiled for each condition.
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| Contributor(s) |
Jin X, Zhang XH, Massague J |
| Citation(s) |
23993096 |
| |
| Submission date |
Jan 04, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Xin Jin |
| E-mail(s) |
jinx@mskcc.org
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| Phone |
6468882540
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| Organization name |
Memorial Sloan-Kettering Cancer Center
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| Department |
Cancer Biology and Genetics
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| Street address |
417 East 68th Street
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA185391 |
| SRA |
SRP017788 |
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