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Series GSE43028 Query DataSets for GSE43028
Status Public on Dec 09, 2013
Title Small and large RNA sequencing of a Kras G12D mouse model of lung adenocarcinoma
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Mice bearing a G12D activating mutation in Kras consistently develop lung adenocarcinomas in a manner analogous to humans. By performing small and large RNA sequencing on KrasG12D tumors from F1 hybrid mice we were able to identify genes and microRNAs differentially expressed in these tumor samples. Quantification of reads that cover single nucleotide polymorphisms that distinguish between the parental mouse strains enabled an analysis of allele specific expression and imprinting status in these tumors.
Overall design mRNA and small RNA fractions of mouse lungs and lung adenocarcinomas were deep sequenced in triplicate
Contributor(s) Valdmanis PN, Kay MA
Citation(s) 24317514
Submission date Dec 19, 2012
Last update date May 15, 2019
Contact name Paul Valdmanis
Phone 206-221-8059
Organization name University of Washington
Department Medicine
Lab Paul Valdmanis
Street address 1705 NE Pacific St, HSB J-309
City Seattle
State/province WA
ZIP/Postal code 98115-7720
Country USA
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (12)
GSM1055270 normal_lung_rep1_total RNA_total RNA
GSM1055271 normal_lung_rep2_total RNA_total RNA
GSM1055272 normal_lung_rep3_total RNA_total RNA
BioProject PRJNA184072
SRA SRP017615

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Supplementary file Size Download File type/resource
GSE43028_RNAseq_FPMK.txt.gz 567.6 Kb (ftp)(http) TXT
GSE43028_microRNA_reads_per_million.txt.gz 7.2 Kb (ftp)(http) TXT
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