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Series GSE42912 Query DataSets for GSE42912
Status Public on Jan 14, 2013
Title Nurr1 maintains fiber integrity and nuclear-encoded mitochondrial gene expression in dopamine neurons
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Nurr1 (Nr4a2, nuclear receptor subfamily 4 group A member 2) is needed for the development of ventral midbrain dopaminergic neurons, and has been associated with Parkinson's disease. We used mice where the Nurr1 gene is ablated by tamoxifen treatment selectively in dopaminergic neurons. As a control, we used tamoxifen-treated mice where Nurr1 is not ablated. By laser microdissection of neurons selected by their TH1 (Th1l, TH1-like homolog) gene expression, we selected dopaminergic neurons for RNA extraction and high-throughput mRNA sequencing, in order to identify genes regulated by Nurr1. We found the main functional category of Nurr1-regulated genes are the nuclear-encoded mitochondrial genes.
Overall design Dopaminergic neurons with or without Nurr1 knocked out.
TH-positive neurons were laser capture microdissected from cryostat coronal sections of the midbrain.
Contributor(s) Kadkhodaei B, Ramsköld D, Perlmann T
Citation(s) 23341612
Submission date Dec 13, 2012
Last update date May 15, 2019
Contact name Daniel Ramsköld
Organization name Karolinska Institutet
Lab Rickard Sandberg's group ( (2008-2014, 2018-); Rheumatology unit (2014-2018)
Street address Nobels väg 3
City Stockholm
ZIP/Postal code 17177
Country Sweden
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (15)
GSM1053278 Substantia nigra dopaminergic neurons, Nurr1 cKO 1
GSM1053279 Substantia nigra dopaminergic neurons, Nurr1 cKO 2
GSM1053280 Substantia nigra dopaminergic neurons, Nurr1 cKO 3
BioProject PRJNA183857
SRA SRP017560

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Supplementary file Size Download File type/resource
GSE42912_RAW.tar 988.2 Mb (http)(custom) TAR (of BED, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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