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Series GSE42852 Query DataSets for GSE42852
Status Public on Mar 14, 2013
Title PRC1 coordinates timing of sexual differentiation of female primordial germ cells (RNA-Seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In mammals, sex differentiation of primordial germ cells (PGCs) is determined by extrinsic cues from the environment1. In female PGCs, expression of Stimulated by retinoic acid 8 (Stra8) and meiosis are induced in response to retinoic acid (RA) provided by the mesonephroi2-4. Given the widespread role of RA signaling during development8,9, the molecular mechanism specifying the competence of PGCs to timely express Stra8 and enter meiosis are unknown2,10. Here we identify gene dosage dependent roles in PGC development for Ring1 and Rnf2, two central components of the Polycomb Repressive Complex 1 (PRC1)11,13. Both paralogs are essential for PGC development between day 10.5 and 11.5 of gestation. Rnf2 is subsequently required in female PGCs for maintaining high levels of Oct4 and Nanog expression6, and for preventing premature induction of meiotic gene expression and entry into meiotic prophase. Chemical inhibition of RA signaling partially suppresses precocious Oct4 down-regulation and Stra8 activation in Rnf2-deficient female PGCs. Chromatin immunoprecipitation analyses show that Stra8 is a direct target of PRC1 and PRC2 in PGCs. These data demonstrate the importance of PRC1 gene dosage in PGC development and in coordinating the timing of sex differentiation of female PGCs by antagonizing extrinsic RA signaling.
Overall design Gene expression of mouse primordial germ cells was analysed using RNAseq method. Primodial germ cells were purified from embryos carrying Oct4(-delta-PE)-GFP transgene by FACS.
Contributor(s) Yokobayashi S, Kohler H, Nestorov P, Jacobeit K, Roloff TC, Peters AH
Citation(s) 23486062
Submission date Dec 11, 2012
Last update date May 15, 2019
Contact name Tim Roloff
Organization name FMI
Department Functional Genomics
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (3)
GSM1051316 Ring1-delta-Rnf2+_E11.5_male_rep1
GSM1051317 Ring1-delta-Rnf2+_E11.5_male_rep2
GSM1051318 Ring1-delta-Rnf2-delta-_E11.5_male_rep1
BioProject PRJNA183639
SRA SRP017513

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Supplementary file Size Download File type/resource
GSE42852_RAW.tar 816.3 Mb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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