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Series GSE42817 Query DataSets for GSE42817
Status Public on Apr 14, 2015
Title Characterization of IGF1 effects on the transcriptome of normal and irradiated Sox9-EGFP cell populations
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcriptional profiling of intestinal epithelial cells expressing either Negative, Sublow, Low or High levels of the Sox9-EGFP reporter transgene FACS-isolated from jejunum of non-irradiated mice or at 5 days after 14Gy abdominal irradiation. In both groups, mice were treated for 5 consecutive days with either IGF1 or vehicle via mini-pumps (Alzet 1007D, IGF1 at 10mg/ml) implanted subcutaneously immediately following radiation.
Overall design 4 distinct cell populations FACS-isolated based on levels of expression of the Sox9-EGFP reporter transgene (Sox9-EGFP Negative, Sublow, Low and High cells).
4 conditions: Non-irradiated/vehicle vs Non-irradiated/IGF1 vs Irradiated/vehicle vs Irradiated/IGF1.
Biological replicates: 7 independent non-irradiated mice treated with vehicle - 3 independent non-irradiated mice treated for 5 days with IGF1 - 3 independent irradiated mice studied at day 5 post-irradiation treated with vehicle - 3 independent irradiated mice studied at day 5 post-irradiation treated for 5 days with IGF1.
Contributor(s) Lund PK, Van Landeghem L, Mah AT
Citation(s) 25837582
Submission date Dec 10, 2012
Last update date Jan 19, 2018
Contact name P. Kay Lund
Phone 919 966 1490
Fax 919 966 6927
Organization name UNC at Chapel Hill
Department Cell and Molecular Physiology
Lab Lund
Street address 6336 MBRB CB 7545 111 Mason Farm Road
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7545
Country USA
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (72)
GSM1050481 Non-irradiated/vehicle Sox9-EGFP Negative rep 1
GSM1050482 Non-irradiated/vehicle Sox9-EGFP Negative rep 2
GSM1050483 Non-irradiated/vehicle Sox9-EGFP Negative rep 3
BioProject PRJNA183489

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42817_RAW.tar 538.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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