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Series GSE42667 Query DataSets for GSE42667
Status Public on Aug 12, 2013
Title Effect of cigarette smoke on gene expression in mammary epithelial cells [gene expression]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Recent epidemiological studies demonstrate that both active and involuntary exposure to tobacco smoke increases the risk of breast cancer. Little is known, however, about the molecular mechanisms by which tobacco smoke contributes to breast carcinogenesis. To investigate these mechanisms we have analyzed gene expression and methylation in MCF 10A mammary epithelial cells chronically exposed to aqueous cigarette smoke extract (CSE).
Overall design CSE was prepared weekly and added to the cell cultures at a concentration equivalent to 0.001 cigarettes/ml. Two clones were isolated after 13 weeks of treatment and expanded in the same concentration of CSE for 8 additional weeks. Mock-treated samples were prepared in parallel.
Contributor(s) Zahnow CA, Di Cello F
Citation(s) 23919753
Submission date Dec 02, 2012
Last update date Feb 22, 2018
Contact name Francescopaolo Di Cello
Organization name The Johns Hopkin University School of Medicine
Street address 1650 Orleans Street
City Baltimore
State/province MD
ZIP/Postal code 21287
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (2)
GSM1047679 MCF10A_CSE_21weeks_clone 1
GSM1047680 MCF10A_CSE_21weeks_clone 2
This SubSeries is part of SuperSeries:
GSE42668 Effect of cigarette smoke on gene expression in mammary epithelial cells
BioProject PRJNA182598

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Supplementary file Size Download File type/resource
GSE42667_RAW.tar 37.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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