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| Status |
Public on Aug 12, 2013 |
| Title |
Effect of cigarette smoke on gene expression in mammary epithelial cells [gene expression] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Recent epidemiological studies demonstrate that both active and involuntary exposure to tobacco smoke increases the risk of breast cancer. Little is known, however, about the molecular mechanisms by which tobacco smoke contributes to breast carcinogenesis. To investigate these mechanisms we have analyzed gene expression and methylation in MCF 10A mammary epithelial cells chronically exposed to aqueous cigarette smoke extract (CSE).
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| Overall design |
CSE was prepared weekly and added to the cell cultures at a concentration equivalent to 0.001 cigarettes/ml. Two clones were isolated after 13 weeks of treatment and expanded in the same concentration of CSE for 8 additional weeks. Mock-treated samples were prepared in parallel.
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| Contributor(s) |
Zahnow CA, Di Cello F |
| Citation(s) |
23919753 |
| |
| Submission date |
Dec 02, 2012 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Francescopaolo Di Cello |
| E-mail(s) |
fdicello@hotmail.com
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| Organization name |
The Johns Hopkin University School of Medicine
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| Street address |
1650 Orleans Street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE42668 |
Effect of cigarette smoke on gene expression in mammary epithelial cells |
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| Relations |
| BioProject |
PRJNA182598 |
