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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 24, 2013 |
| Title |
High-throughput single cell gene expression analysis in primary blood stem and progenitor cells identifies new links between key transcriptional regulators |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Cellular decision-making is mediated by a complex interplay of external stimuli with the intracellular environment, in particular transcription factor regulatory networks. Here we have determined the expression of a network of 18 key haematopoietic transcription factors (TFs) in 597 single primary blood stem and progenitor cells isolated from mouse bone marrow. We demonstrate that different stem/progenitor populations are characterised by distinctive TF expression states, and through comprehensive bioinformatic analysis reveal positively and negatively correlated TF pairings, including previously unrecognised relationships between Gata2, Gfi1 and Gfi1b. Validation using transcriptional and transgenic assays confirmed direct regulatory interactions consistent with a novel regulatory triad in immature blood stem cells, where Gata2 may function to modulate cross-inhibition between Gfi1 and Gfi1b. Single cell expression profiling therefore identifies network states and allows reconstruction of network hierarchies involved in controlling stem cell fate choices, and provides a blueprint for studying both normal development and human disease.
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| Overall design |
Examination of Gata2 and Gfi1 binding patterns in murine mast cells
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| Contributor(s) |
Moignard V, Macaulay I, Swiers G, Buettner F, Schuette J, Calero-Nieto F, Kinston S, Joshi A, Hannah R, Theis F, Jacobsen SE, de Bruijn M, Gottgens B |
| Citation(s) |
23524953 |
| Submission date |
Nov 26, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Rebecca Louise Hannah |
| E-mail(s) |
rlh60@cam.ac.uk, rebeccahannah@live.co.uk
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| Organization name |
University of Cambridge
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| Department |
Haematology
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| Street address |
Addenbrookes Hospital, Hills Road
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA182188 |
| SRA |
SRP017312 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42518_RAW.tar |
200.6 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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