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Series GSE42518 Query DataSets for GSE42518
Status Public on Apr 24, 2013
Title High-throughput single cell gene expression analysis in primary blood stem and progenitor cells identifies new links between key transcriptional regulators
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Cellular decision-making is mediated by a complex interplay of external stimuli with the intracellular environment, in particular transcription factor regulatory networks. Here we have determined the expression of a network of 18 key haematopoietic transcription factors (TFs) in 597 single primary blood stem and progenitor cells isolated from mouse bone marrow. We demonstrate that different stem/progenitor populations are characterised by distinctive TF expression states, and through comprehensive bioinformatic analysis reveal positively and negatively correlated TF pairings, including previously unrecognised relationships between Gata2, Gfi1 and Gfi1b. Validation using transcriptional and transgenic assays confirmed direct regulatory interactions consistent with a novel regulatory triad in immature blood stem cells, where Gata2 may function to modulate cross-inhibition between Gfi1 and Gfi1b. Single cell expression profiling therefore identifies network states and allows reconstruction of network hierarchies involved in controlling stem cell fate choices, and provides a blueprint for studying both normal development and human disease.
Overall design Examination of Gata2 and Gfi1 binding patterns in murine mast cells
Contributor(s) Moignard V, Macaulay I, Swiers G, Buettner F, Schuette J, Calero-Nieto F, Kinston S, Joshi A, Hannah R, Theis F, Jacobsen SE, de Bruijn M, Gottgens B
Citation(s) 23524953
Submission date Nov 26, 2012
Last update date May 15, 2019
Contact name Rebecca Louise Hannah
Organization name University of Cambridge
Department Haematology
Street address Addenbrookes Hospital, Hills Road
City Cambridge
ZIP/Postal code CB2 0XY
Country United Kingdom
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1041378 Gata2
GSM1041379 Gfi1
BioProject PRJNA182188
SRA SRP017312

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Supplementary file Size Download File type/resource
GSE42518_RAW.tar 200.6 Mb (http)(custom) TAR (of BEDGRAPH)
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Raw data are available in SRA
Processed data provided as supplementary file

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