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Status |
Public on Apr 15, 2006 |
Title |
cDNA microarray analysis of human skeletal muscle after eccentric exercise |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
We examined global mRNA expression using cDNA microarrays in skeletal muscle of humans before, and 3h and 48h after 300 maximal eccentric contractions. Keywords: Time course
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Overall design |
Healthy, non-trained university-aged subjects performed 300 single leg maximal eccentric contractions. Skeletal muscle biopsies were taken from the vastus lateralis before, 3h and 48h after the exercise bout. Total RNA was extracted, amplified, reverse transcribed, and cDNA was analyzed on a custom made cDNA microarray. Four subjects were analyzed, and samples were not pooled between subjects (i.e. individual microarrays were used for baseline vs. 3H and baseline vs. 48h for EACH subject; repeated measures design).
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Contributor(s) |
Mahoney DJ, Parise G, Melov S, Safdar A, Fu M, Payne ET, Tarnopolsky MA |
Citation missing |
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Submission date |
Feb 15, 2006 |
Last update date |
Mar 17, 2012 |
Contact name |
Douglas Joseph Mahoney |
E-mail(s) |
doug@mgcheo.med.uottawa.ca
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Phone |
613 738 3927
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Fax |
613 738 4833
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Organization name |
CHEO Research Institute
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Department |
Apoptosis Research Center
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Street address |
401 Smyth Rd
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City |
Ottawa |
State/province |
ON |
ZIP/Postal code |
K1H 8L1 |
Country |
Canada |
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Platforms (1) |
GPL3457 |
Buck Institute Human 8k cDNA microarray |
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Samples (7)
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This SubSeries is part of SuperSeries: |
GSE4252 |
cDNA microarray analysis of human skeletal muscle after eccentric or endurance exercise |
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Relations |
BioProject |
PRJNA104621 |