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| Status |
Public on Nov 22, 2012 |
| Title |
Gene expression profile of L428 cells conditional expressing PU.1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
PU.1 is an Ets family transcription factor that is essential for the differentiation of both myeloid and lymphoid cells. PU.1 is down-regulated in classical Hodgkin lymphoma cells via methylation of the PU.1 promoter. To evaluate whether down-regulation of PU.1 is essential for the growth of cHL cells, we generated L428 derived cell lines conditionally express PU.1 by tet-off system (designated L428tetPU.1). Conditonally expressed PU.1 by tetracycline removal induced complete growth arrest and apoptosis in L428 cells. To elucidate the mechanisms underlying cell cycle arrest and apoptosis induced by PU.1, we compared gene expression profiles of L428tetPU.1 cells 0, 1 and 3 days after PU.1 induction, by DNA microarray.
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| Overall design |
We extracted total RNA from L428tetPU.1 cells 0, 1 and 3 days after PU.1 induction by tetracycline removal. We compared gene expression profiles of KL428tetPU.1 cells 0, 1 and 3 days after PU.1 induction using DNA microarray analysis. 4 independent experiments were performed with each RNA samples.
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| Contributor(s) |
Yuki H, Ueno S, Tatetsu H, Niiro H, Iino T, Endo S, Kawano Y, Komohara Y, Takeya M, Hata H, Okada S, Watanabe T, Akashi K, Mitsuya H, Okuno Y |
| Citation(s) |
23212521 |
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| Submission date |
Nov 21, 2012 |
| Last update date |
Feb 18, 2014 |
| Contact name |
Yutaka Okuno |
| E-mail(s) |
yokuno@gpo.kumamoto-u.ac.jp
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| Phone |
+81-96-373-5156
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| Organization name |
Kumamoto university of Medicine
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| Department |
Department of Hematology
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| Street address |
1-1-1 Honko, Chuo Ku
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| City |
Kumamoto |
| State/province |
Kumamoto |
| ZIP/Postal code |
860-8556 |
| Country |
Japan |
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| Platforms (1) |
| GPL13376 |
Illumina HumanWG-6 v2.0 expression beadchip |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE42441 |
PU.1 is a potent tumor suppressor in classical Hodgkin lymphoma cells |
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| Relations |
| BioProject |
PRJNA181980 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42437_RAW.tar |
12.0 Mb |
(http)(custom) |
TAR |
| GSE42437_non-normalized_data.txt.gz |
728.9 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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