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| Status |
Public on Mar 15, 2015 |
| Title |
Gene expression profiles of parental and chemotherapy-resistant colon cancer cell lines. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of this study was to identify biomarkers of drug response and investigate mechanisms of drug resistance to the two chemotherapeutic drugs oxaliplatin and irinotecan (active metabolite SN-38) in colorectal cancer, using a cell-based model system of drug-sensitive parental colon cancer cell lines and sub-cell lines with acquired drug resistance.
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| Overall design |
Three human colon cancer cell lines (HCT116, HT29 and LoVo) were exposed in vitro to gradually increasing drug (oxaliplatin or SN-38) concentrations for about nine months, generating sub-cell lines with acquired resistance.
Gene expression profiles of the parental and resistant cell lines cultured in drug-free medium for two to three weeks were obtained (each in triplicate) and compared to identify gene expression changes associated to chemotherapy resistance.
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| Contributor(s) |
Jensen NF, Stenvang J, Belling KC, Do KN, Gupta R, Rømer MU, Brünner N |
| Citation(s) |
25759163 |
| |
| Submission date |
Nov 19, 2012 |
| Last update date |
Mar 17, 2015 |
| Contact name |
Niels Frank Jensen |
| Organization name |
University of Copenhagen
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| Department |
Faculty of Health and Medical Sciences
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| Lab |
Pathobiology
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| Street address |
Dyrlaegevej 88, 1.
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| City |
Frederiksberg C |
| ZIP/Postal code |
1870 |
| Country |
Denmark |
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| Platforms (1) |
| GPL16297 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Agilent Systematic Name, collapsed probe, version) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA181264 |
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