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Series GSE42339 Query DataSets for GSE42339
Status Public on Aug 01, 2013
Title RNA-Seq expression data from the reproductive tracts of WT, Hoxa91011, and Hoxd91011 mutant mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Hox genes are key regulators of development. In mammals, the study of these genes is greatly confounded by their large number, overlapping functions, and their interspersed shared enhancers. In this report, we describe a novel recombineering strategy that was used to introduce simultaneous frameshift mutations into the flanking Hoxa9, Hoxa10, and Hoxa11 genes, as well as their paralogs on the HoxD cluster. The resulting mutant mice displayed dramatic homeotic transformations of the reproductive tracts, with uterus anteriorized towards oviduct and the vas deferens anteriorized towards epididymis. The Hoxa9,10,11 mutant mice provided a sensitized genetic background that allowed the discovery of Hoxd9,10,11 reproductive tract patterning function. Both shared and distinct Hox functions were defined. The HoxD genes played a crucial role in the regulation of the uterine immune function. Non-coding nonpolyadenylated RNAs were among the key Hox targets. In addition we observed a surprising anti-dogmatic posteriorization of the uterine epithelium.
Overall design Reproductive tracts were collected from WT and Hox mutant mice (n=3/genotype) aged 3-7 months in order to characterize the molecular changes caused by mutation of Hoxa9,10,11 and Hoxd9,10,11. Female mice were staged and collected in diestrus.
Contributor(s) Raines AM, Adam M, Magella B, Meyer SE, Grimes HL, Dey SK, Potter SS
Citation(s) 23760953
Submission date Nov 16, 2012
Last update date May 15, 2019
Contact name Steve Potter
Organization name Cincinnati Children's Hospital Medical Center
Department Developmental Biology
Street address 3333 Burnet Ave.
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (12)
GSM1038105 WT uterus, pooled from n=3
GSM1038106 Hoxa9,10,11-/- uterus, pooled from n=3
GSM1038107 Hoxd9,10,11-/- uterus, pooled from n=3
BioProject PRJNA181109
SRA SRP017207

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42339_RAW.tar 8.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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