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| Status |
Public on May 01, 2013 |
| Title |
Expression data from E. faecalis Fsr mutants during GBAP induction |
| Platform organism |
Enterococcus faecalis |
| Sample organism |
Enterococcus faecalis V583 |
| Experiment type |
Expression profiling by array
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| Summary |
To rigorously characterize the Fsr regulon, we compared gene expression in V583 isogenic mutants in Fsr genes and each of the Fsr-regulated protease genes using microarrays and purified GBAP, the quorum-sensing molecule. We used microarrays to identified the genes that are regulated directly by Fsr Quorum sensing system.
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| Overall design |
E. faecalis V583 cells were collected at 0 minutes and 10 minutes after GBAP addition, for RNA extraction and hybridization on Affymetrix microarrays. The cells were collected when the number of the cells are ideal for Quorum sensing activation. This procedure was also made without GBAP addition. For each condition were made replicates.
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| Contributor(s) |
Teixeira N, Varahan S, Gorman MJ, Palmer KL, Yokohata R, Nakayama J, Hancock LE, Gilmore MS, Lopes MS |
| Citation(s) |
23734216 |
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| Submission date |
Nov 05, 2012 |
| Last update date |
Nov 13, 2013 |
| Contact name |
Neuza Teixeira |
| E-mail(s) |
neuzat@itqb.unl.pt
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| Organization name |
ITQB
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| Lab |
Stress by Antibiotics and Virulence of Enterococci
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| Street address |
Av. da República, Estação Agronómica Nacional
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| City |
Oeiras |
| ZIP/Postal code |
2780-157 |
| Country |
Portugal |
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| Platforms (1) |
| GPL16245 |
[Gilmorea520187F] Affymetrix Custom Array - Gilmore SLARE E. faecalis |
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| Samples (20)
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| GSM1030936 |
FsrB mutant after 10 min of GBAP addition, biological rep1 |
| GSM1030937 |
FsrB mutant after 10 min of GBAP addition, biological rep2 |
| GSM1030938 |
FsrB mutant after 0 min of GBAP addition, biological rep1 |
| GSM1030939 |
FsrB mutant after 0 min of GBAP addition, biological rep2 |
| GSM1030940 |
∆fsrB∆gelE mutant after 10 min of GBAP addition, biological rep1 |
| GSM1030941 |
∆fsrB∆gelE mutant after 10 min of GBAP addition, biological rep2 |
| GSM1030942 |
∆fsrB∆gelE mutant after 0 min of GBAP addition, biological rep1 |
| GSM1030943 |
∆fsrB∆gelE mutant after 0 min of GBAP addition, biological rep2 |
| GSM1030944 |
∆fsrB∆sprE mutant after 10 min of GBAP addition, biological rep1 |
| GSM1030945 |
∆fsrB∆sprE mutant after 10 min of GBAP addition, biological rep2 |
| GSM1030946 |
∆fsrB∆sprE mutant after 0 min of GBAP addition, biological rep1 |
| GSM1030947 |
∆fsrB∆sprE mutant after 0 min of GBAP addition, biological rep2 |
| GSM1030948 |
∆fsrB∆gelE∆sprE mutant after 10 min of GBAP addition, biological rep1 |
| GSM1030949 |
∆fsrB∆gelE∆sprE mutant after 10 min of GBAP addition, biological rep2 |
| GSM1030950 |
∆fsrB∆gelE∆sprE mutant after 0 min of GBAP addition, biological rep1 |
| GSM1030951 |
∆fsrB∆gelE∆sprE mutant after 0 min of GBAP addition, biological rep2 |
| GSM1030952 |
FsrB mutant after 10 min, without GBAP addition, biological rep1 |
| GSM1030953 |
FsrB mutant after 10 min, without GBAP addition, biological rep2 |
| GSM1030954 |
FsrB mutant after 0 min, without GBAP addition, biological rep1 |
| GSM1030955 |
FsrB mutant after 0 min, without GBAP addition, biological rep2 |
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| Relations |
| BioProject |
PRJNA178964 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42036_RAW.tar |
11.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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