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Status |
Public on Feb 17, 2006 |
Title |
Zebrafish microRNA miR-430 promotes deadenylation and clearance of maternal mRNAs |
Organism |
Danio rerio |
Experiment type |
Expression profiling by array
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Summary |
MicroRNAs comprise 1-3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. Using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target mRNAs. Targets are highly enriched for maternal mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis. Keywords: Dicer, MZdicer, miR-430, miRNA target, maternal, zygotic
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Overall design |
As a first step towards the identification of miR-430 targets, we compared the rate of mRNA degradation in wild type and maternal-zygotic (MZ) dicer mutant embryos, which are deficient in miRNA processing. We found that the rate of degradation of a target mRNA with partial complementarity to miR-430 is significantly reduced in MZdicer mutants. Providing miR-430 duplexes to MZdicer mutants restores the rate of target decay. These results indicate that miR-430 enhances the decay of target mRNAs.
To identify miR-430 in vivo targets, we compared the mRNA expression profile of embryos that were wild-type, MZdicer, or MZdicer rescued with miR-430 (MZdicer+miR-430). This analysis identified ~600 genes that were upregulated in MZdicer, but not in MZdicer+miR-430.
We have also analyzed the expression profile of the genes in the array at three different time points, one maternal stage(16 cell), and two Zygotic stages 5 hours and 9 hours
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Contributor(s) |
Giraldez AJ, Mishima Y, Rihel J, Grocock RJ, Van Dongen S, Inoue K, Enright AJ, Schier AF |
Citation(s) |
16484454 |
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Submission date |
Feb 07, 2006 |
Last update date |
Jan 25, 2018 |
Contact name |
Antonio J. Giraldez |
E-mail(s) |
antonio.giraldez@yale.edu
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Phone |
203 785 5423
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Organization name |
Yale University
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Department |
Genetics
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Lab |
Giraldez Lab
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Street address |
333 Cedar Street
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City |
New Haven |
State/province |
CT |
ZIP/Postal code |
06510 |
Country |
USA |
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Platforms (1) |
GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
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Samples (15)
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GSM95611 |
Zebrafish Embyro_16cell stage_Rep1 |
GSM95612 |
Zebrafish Embryo_16 cell stage_Rep2 |
GSM95613 |
Zebrafish Embryo_50% epiboly |
GSM95614 |
Zebrafish_MZ Dicer_miR-430 injected_Rep1 |
GSM95615 |
Zebrafish_MZ Dicer_miR-430 injected_Rep2 |
GSM95616 |
Zebrafish_MZ Dicer_miR-430 injected_Rep3 |
GSM95617 |
Zebrafish_MZ Dicer_Rep1 |
GSM95618 |
Zebrafish_MZ Dicer_Rep2 |
GSM95619 |
Zebrafish_MZ Dicer_Rep3 |
GSM95620 |
Zebrafish_Wild Type_miR-430 injected_Rep1 |
GSM95621 |
Zebrafish_Wild Type_miR-430 injected_Rep2 |
GSM95622 |
Zebrafish_Wild Type_miR-430 injected_Rep3 |
GSM95623 |
Zebrafish_Wild Type_Rep1 |
GSM95624 |
Zebrafish_Wild Type_Rep2 |
GSM95625 |
Zebrafish_Wild Type_Rep3 |
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Relations |
BioProject |
PRJNA95055 |
Supplementary file |
Size |
Download |
File type/resource |
GSE4201_RAW.tar |
51.5 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
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