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Series GSE41937 Query DataSets for GSE41937
Status Public on Jan 07, 2013
Title Dual Functions of TAF7L in Adipocyte Differentiation
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary The diverse transcriptional mechanisms governing cellular differentiation and development of mammalian tissue remains poorly understood. Here we report that TAF7L, a paralogue of TFIID subunit TAF7, is enriched in adipocytes and mouse white fat tissue (WAT). Depletion of TAF7L reduced adipocyte-specific gene expression and compromised adipocyte differentiation as well as WAT development. Ectopic expression of TAF7L in myoblasts reprograms these muscle precursors into adipocytes upon induction. Genome-wide mRNA-seq expression profiling and ChIP-seq binding studies confirmed that TAF7L is required for activating adipocyte-specific genes via a dual mechanism wherein it interacts with PPARγ at enhancers and TBP/Pol II at core promoters. In vitro binding studies confirmed that TAF7L forms complexes with both TBP and PPARγ. These findings suggest that TAF7L plays an integral role in adipocyte gene expression by targeting enhancers as a cofactor for PPARγ and promoters as a component of the core transcriptional machinery.
Overall design Genome-wide mapping of TAF7L and additional factors, and mRNA-seq expression profiling prior to and following mouse adipocyte differentiation.
Contributor(s) Zhou H, Kaplan T, Li Y, Grubisic I, Zhang Z, Wang PJ, Eisen MB, Tjian R
Citation(s) 23326641
Submission date Oct 31, 2012
Last update date May 15, 2019
Contact name Tommy Kaplan
Organization name Hebrew University
Department School of Computer Science and Engineering
Street address Givat Ram Campus
City Jerusalem
ZIP/Postal code 91904
Country Israel
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (15)
GSM1030539 IgG ChIP-seq (Adipocytes)
GSM1030540 IgG ChIP-seq (10T1/2)
GSM1030541 PolII ChIP-seq (Adipocytes)
BioProject PRJNA178771
SRA SRP017034

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Supplementary file Size Download File type/resource
GSE41937_Adipose_mRNA_ChIP.txt.gz 2.8 Mb (ftp)(http) TXT
GSE41937_Adipose_mRNA_ChIP.xlsx.gz 12.3 Mb (ftp)(http) XLSX
GSE41937_RAW.tar 5.0 Mb (http)(custom) TAR (of XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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